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Antimicrobial Agents and Chemotherapy, August 1999, p. 2087-2089, Vol. 43, No. 8
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Contribution of kasA Analysis to Detection of Isoniazid-Resistant Mycobacterium tuberculosis in Singapore

Ann S. G. Lee,1,* Irene H. K. Lim,2 Lynn L. H. Tang,2 Amalio Telenti,3 and Sin Yew Wong2

Department of Clinical Research, Ministry of Health, Singapore General Hospital, Singapore 169608,1 and Communicable Disease Centre, Tan Tock Seng Hospital, Singapore 308433,2 Singapore, and Division of Infectious Diseases, Centre Hospitalier Universitaire Vaudois, 1011 Lausanne, Switzerland3

Received 12 November 1998/Returned for modification 19 March 1999/Accepted 27 May 1999

Genotypic analysis of resistance to isoniazid (INH) in Mycobacterium tuberculosis is complex due to the various genes potentially involved. Mutations in ketoacyl acyl carrier protein synthase (encoded by kasA) were present in 16 of 160 (10%) INH-resistant isolates (R121K [n = 1], G269S [n = 3], G312S [n = 11], G387D [n = 1]). However, G312S was also present in 6 of 32 (19%) susceptible strains. kasA analysis contributed marginally to the performance of INH genotypic testing in Singapore. The significance of kasA polymorphisms in INH resistance should be carefully established.

* Corresponding author. Mailing address: Department of Clinical Research, Block 6, Level 6, Singapore General Hospital, Outram Road, Singapore 169608, Singapore. Phone: 65-3213730. Fax: 65-2257796. E-mail: gcrlsg{at}sgh.gov.sg.

Antimicrobial Agents and Chemotherapy, August 1999, p. 2087-2089, Vol. 43, No. 8
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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