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Antimicrobial Agents and Chemotherapy, September 1999, p. 2311-2313, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antimicrobial Susceptibility Testing of Chlamydia trachomatis Using a Reverse Transcriptase PCR-Based Method

N. A. Cross,1 D. J. Kellock,2 G. R. Kinghorn,2 M. Taraktchoglou,1 E. Bataki,1 K. M. Oxley,1 P. M. Hawkey,3 and A. Eley1,*

Division of Molecular and Genetic Medicine, University of Sheffield Medical School,1 and Department of Genitourinary Medicine, Royal Hallamshire Hospital,2 Sheffield S10 2RX, and Department of Microbiology, University of Leeds, Leeds LS2 9JT,3 United Kingdom

Received 16 November 1998/Returned for modification 20 March 1999/Accepted 23 June 1999

The conventional method for antimicrobial susceptibility testing of Chlamydia trachomatis is subjective and potentially misleading. We have developed a reverse transcriptase PCR (RT-PCR)-based method which is more sensitive and less subjective than the conventional method. Using 16 strains of C. trachomatis in triplicate assays, we found the RT-PCR method consistently more sensitive than the conventional technique for all eight antimicrobials tested, with resultant MICs determined by RT-PCR ranging from 1.6-fold higher (erythromycin) to >= 195-fold higher (amoxicillin).

* Corresponding author. Mailing address: Division of Molecular and Genetic Medicine, University of Sheffield Medical School, Beech Hill Rd., Sheffield S10 2RX, United Kingdom. Phone: (44) 114-2712335. Fax (44) 114-2739926. E-mail: a.r.eley{at}sheffield.ac.uk.

Antimicrobial Agents and Chemotherapy, September 1999, p. 2311-2313, Vol. 43, No. 9
0066-4804/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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