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Antimicrobial Agents and Chemotherapy, October 2000, p. 2908-2910, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Methionine Interference in Rapamycin Production Involves Repression of Demethylrapamycin Methyltransferase and S-Adenosylmethionine Synthetase

Wan-Seop Kim, Yi Wang, Aiqi Fang, and Arnold L. Demain*

Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 27 March 2000/Returned for modification 8 June 2000/Accepted 28 June 2000

In a chemically defined medium, L-methionine decreased production of rapamycin and increased that of demethylrapamycin. Growth with L-methionine yielded cells with a lower ability to convert demethylrapamycin to rapamycin and decreased the level of S-adenosylmethionine synthetase and S-adenosylmethionine. Thus, methionine represses at least one methyltransferase of rapamycin biosynthesis and S-adenosylmethionine synthetase.

* Corresponding author. Mailing address: 68-223, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139. Phone: (617) 253-1711. Fax: (617) 253-8699. E-mail: demain{at}mit.edu.

Antimicrobial Agents and Chemotherapy, October 2000, p. 2908-2910, Vol. 44, No. 10
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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  • Okamoto, S., Lezhava, A., Hosaka, T., Okamoto-Hosoya, Y., Ochi, K. (2003). Enhanced Expression of S-Adenosylmethionine Synthetase Causes Overproduction of Actinorhodin in Streptomyces coelicolor A3(2). J. Bacteriol. 185: 601-609 [Abstract] [Full Text]  


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