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Antimicrobial Agents and Chemotherapy, November 2000, p. 3035-3039, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

SME-Type Carbapenem-Hydrolyzing Class A beta -Lactamases from Geographically Diverse Serratia marcescens Strains

Anne Marie Queenan,1,* Carlos Torres-Viera,2,3 Howard S. Gold,2,3 Yehuda Carmeli,2,3 George M. Eliopoulos,2,3 Robert C. Moellering Jr.,2,3 John P. Quinn,4 Janet Hindler,5 Antone A. Medeiros,6 and Karen Bush1

The R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 088691; Beth Israel Deaconess Medical Center2 and Harvard Medical School,3 Boston, Massachusetts 02115; University of Illinois, Chicago, Illinois 606144; University of California at Los Angeles Medical Center, Los Angeles, California 900245; and Miriam Hospital, Brown University, Providence, Rhode Island 029066

Received 7 March 2000/Returned for modification 8 June 2000/Accepted 10 August 2000

Three sets of carbapenem-resistant Serratia marcescens isolates have been identified in the United States: 1 isolate in Minnesota in 1985 (before approval of carbapenems for clinical use), 5 isolates in Los Angeles (University of California at Los Angeles [UCLA]) in 1992, and 19 isolates in Boston from 1994 to 1999. All isolates tested produced two beta -lactamases, an AmpC-type enzyme with pI values of 8.6 to 9.0 and one with a pI value of approximately 9.5. The enzyme with the higher pI in each strain hydrolyzed carbapenems and was not inhibited by EDTA, similar to the chromosomal class A SME-1 beta -lactamase isolated from the 1982 London strain S. marcescens S6. The genes encoding the carbapenem-hydrolyzing enzymes were cloned in Escherichia coli and sequenced. The enzyme from the Minnesota isolate had an amino acid sequence identical to that of SME-1. The isolates from Boston and UCLA produced SME-2, an enzyme with a single amino acid change relative to SME-1, a substitution from valine to glutamine at position 207. Purified SME enzymes from the U.S. isolates had beta -lactam hydrolysis profiles similar to that of the London SME-1 enzyme. Pulsed-field gel electrophoresis analysis revealed that the isolates showed some similarity but differed by at least three genetic events. In conclusion, a family of rare class A carbapenem-hydrolyzing beta -lactamases first described in London has now been identified in S. marcescens isolates across the United States.

* Corresponding author. Mailing address: The R. W. Johnson Pharmaceutical Research Institute, 1000 Rt. 202, Raritan, NJ 08869. Phone: (908) 704-5515. Fax: (908) 704-3501. E-mail: aqueenan{at}prius.jnj.com.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3035-3039, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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