This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aubry, A.
Right arrow Articles by Cambau, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aubry, A.
Right arrow Articles by Cambau, E.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, November 2000, p. 3133-3136, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibiotic Susceptibility Pattern of Mycobacterium marinum

Alexandra Aubry,1 Vincent Jarlier,1 Sylvie Escolano,2 Chantal Truffot-Pernot,1 and Emmanuelle Cambau1,*

Laboratoire de Bactériologie-Hygiène et Centre National de Référence pour la Surveillance des Infections à Mycobactéries et de leur Résistance aux Antituberculeux,1 and Unité INSERM U436,2 Faculté de Médecine Pitié-Salpêtrière, Paris, France

Received 18 May 2000/Returned for modification 20 July 2000/Accepted 24 August 2000

In vitro activities of 17 antibiotics against 53 clinical strains of Mycobacterium marinum, an atypical mycobacterium responsible for cutaneous infections, were determined using the reference agar dilution method. Rifampin and rifabutin were the most active drugs (MICs at which 90% of the isolates tested were inhibited [MIC90s], 0.5 and 0.6 µg/ml, respectively). MICs of minocycline (MIC90, 4 µg/ml), doxycycline (MIC90, 16 µg/ml), clarithromycin (MIC90, 4 µg/ml), sparfloxacin (MIC90, 2 µg/ml), moxifloxacin (MIC90, 1 µg/ml), imipenem (MIC90, 8 µg/ml), sulfamethoxazole (MIC90, 8 µg/ml) and amikacin (MIC90, 4 µg/ml) were close to the susceptibility breakpoints. MICs of isoniazid, ethambutol, trimethoprim, azithromycin, ciprofloxacin, ofloxacin, and levofloxacin were above the concentrations usually obtained in vivo. For each drug, the MIC50, geometric mean MIC, and modal MIC were very close, showing that all the strains had a similar susceptibility pattern. Percent agreement (within ±1 log2 dilution) between MICs yielded by the Etest method and by the agar dilution method used as reference were 83, 59, 43, and 24% for minocycline, rifampin, clarithromycin, and sparfloxacin, respectively. Reproducibility with the Etest was low, in contrast to that with the agar dilution method. In conclusion, M. marinum is a naturally multidrug-resistant species for which the agar dilution method is more accurate than the Etest for antibiotic susceptibility testing.

* Corresponding author. Mailing address: Faculté de Médecine Pitié-Salpêtrière, 91, Boulevard de l'Hôpital, 75634 Paris Cedex 13, France. Phone: (33) 1 40 77 97 46. Fax: (33) 1 45 82 75 77. E-mail: cambau{at}chups.jussieu.fr.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3133-3136, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lillis, J. V., Winthrop, K. L., White, C. R., Simpson, E. L. (2008). Mycobacterium marinum Presenting as Large Verrucous Plaques on the Lower Extremity of a South Pacific Islander. Am J Trop Med Hyg 79: 166-167 [Abstract] [Full Text]  
  • Stinear, T. P., Seemann, T., Harrison, P. F., Jenkin, G. A., Davies, J. K., Johnson, P. D.R., Abdellah, Z., Arrowsmith, C., Chillingworth, T., Churcher, C., Clarke, K., Cronin, A., Davis, P., Goodhead, I., Holroyd, N., Jagels, K., Lord, A., Moule, S., Mungall, K., Norbertczak, H., Quail, M. A., Rabbinowitsch, E., Walker, D., White, B., Whitehead, S., Small, P. L.C., Brosch, R., Ramakrishnan, L., Fischbach, M. A., Parkhill, J., Cole, S. T. (2008). Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis. Genome Res 18: 729-741 [Abstract] [Full Text]  
  • Haydel, S. E., Remenih, C. M., Williams, L. B. (2008). Broad-spectrum in vitro antibacterial activities of clay minerals against antibiotic-susceptible and antibiotic-resistant bacterial pathogens. J Antimicrob Chemother 61: 353-361 [Abstract] [Full Text]  
  • Griffith, D. E., Aksamit, T., Brown-Elliott, B. A., Catanzaro, A., Daley, C., Gordin, F., Holland, S. M., Horsburgh, R., Huitt, G., Iademarco, M. F., Iseman, M., Olivier, K., Ruoss, S., von Reyn, C. F., Wallace, R. J. Jr., Winthrop, K., on behalf of the ATS Mycobacterial Diseases Subcom, (2007). An Official ATS/IDSA Statement: Diagnosis, Treatment, and Prevention of Nontuberculous Mycobacterial Diseases. Am. J. Respir. Crit. Care Med. 175: 367-416 [Full Text]  
  • Aubry, A., Chosidow, O., Caumes, E., Robert, J., Cambau, E. (2002). Sixty-three Cases of Mycobacterium marinum Infection: Clinical Features, Treatment, and Antibiotic Susceptibility of Causative Isolates. Arch Intern Med 162: 1746-1752 [Abstract] [Full Text]  
  • Braback, M., Riesbeck, K., Forsgren, A. (2002). Susceptibilities of Mycobacteriummarinum to Gatifloxacin, Gemifloxacin, Levofloxacin, Linezolid, Moxifloxacin, Telithromycin, and Quinupristin-Dalfopristin (Synercid) Compared to Its Susceptibilities to Reference Macrolides and Quinolones. Antimicrob. Agents Chemother. 46: 1114-1116 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»