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Antimicrobial Agents and Chemotherapy, November 2000, p. 3144-3149, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

ACI-1 from Acidaminococcus fermentans: Characterization of the First beta -Lactamase in Anaerobic Cocci

J. C. Galán,1 M. Reig,1 A. Navas,2 F. Baquero,1 and J. Blázquez1,*

Servicio de Microbiología, Hospital Ramón y Cajal, Instituto Nacional de Salud (INSALUD), 28034 Madrid,1 and Museo Nacional de Ciencias Naturales, CSIC, 28006 Madrid,2 Spain

Received 9 February 2000/Returned for modification 4 June 2000/Accepted 13 August 2000

Acidaminococcus fermentans belongs to the group of strictly anaerobic gram-negative cocci. All previously described Acidaminococcus strains are susceptible to beta -lactam antibiotics. An A. fermentans strain (RYC-MR95) resistant to penicillin and expanded-spectrum cephalosporin (amoxicillin and cefotaxime MICs, 64 µg/ml) was isolated from a human perianal abscess. A fragment encoding a beta -lactamase from genomic DNA was cloned in Escherichia coli K-12 strain HB101, and the recombinant strain expressed resistance to amoxicillin (MIC, 1,024 µg/ml) and cefotaxime (MIC, 4 µg/ml). Clavulanic acid decreased the MICs to 8 and 0.03 µg/ml, respectively. Analysis of the nucleotide sequence revealed a new class A beta -lactamase, ACI-1. In accordance with its biochemical properties, we propose to assign ACI-1 to functional group 2be. The ACI-1 enzyme (estimated pI 4.3) had <50% amino acid identity with any other class A beta -lactamases, the closest being ROB-1 from Haemophilus influenzae (44%). ACI-1 was closer to class A beta -lactamases from some gram-positive organisms (41 to 44% amino acid identity with Bacillus beta -lactamases) than to most class A enzymes from gram-negative organisms (TEM-1, 24.6%). The aci1 gene had a G+C content of 42.1%, in contrast with 56% G+C content for genomic DNA from A. fermentans, thus suggesting that aci1 may have been obtained by horizontal gene transfer.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Ramón y Cajal, INSALUD, 28034 Madrid, Spain. Phone: 34-91-3368330. Fax: 34-91-3368809. E-mail: jblazquez{at}hrc.insalud.es.

Antimicrobial Agents and Chemotherapy, November 2000, p. 3144-3149, Vol. 44, No. 11
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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