High-Level Fluoroquinolone-Resistant Clinical Isolates of Escherichia coli Overproduce Multidrug Efflux Protein AcrA

doi:10.1128/AAC.44.12.3441-3443.2000

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Antimicrobial Agents and Chemotherapy, December 2000, p. 3441-3443, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

High-Level Fluoroquinolone-Resistant Clinical Isolates of Escherichia coli Overproduce Multidrug Efflux Protein AcrA

Annarita Mazzariol,¹^,² Yutaka Tokue,¹ Tiffany M. Kanegawa,¹ Giuseppe Cornaglia,² and Hiroshi Nikaido¹^,^*

Department of Molecular and Cell Biology, University of California, Berkeley, California,¹ and Department of Pathology, Microbiology Section, University of Verona, Verona, Italy²

Received 30 May 2000/Returned for modification 12 July 2000/Accepted 11 September 2000

Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that thisprotein was overexpressed by $>=$ 170% in 9 of 10 clinical isolatesof Esherichia coli with high-level ciprofloxacin resistance (MICs, $>=$ 32 µg/ml) but not in any of the 15 isolates for which the MICwas $<=$ 1 µg/ml.

^* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Room 229 Stanley Hall, University of California,Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290.E-mail: nhiroshi{at}uclink4.berkeley.edu.

Antimicrobial Agents and Chemotherapy, December 2000, p. 3441-3443, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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