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Antimicrobial Agents and Chemotherapy, December 2000, p. 3444-3446, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of a Divergent vanD-Type Resistance Element from the First Glycopeptide-Resistant Strain of Enterococcus faecium Isolated in Brazil

Libera M. Dalla Costa,1,2 Peter E. Reynolds,3 Helena A. P. H. M. Souza,2 Dilair C. Souza,2 Marie-France I. Palepou,1 and Neil Woodford1,*

Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT,1 and Department of Biochemistry, University of Cambridge CB2 1QW,3 United Kingdom, and Hospital de Clinicas---Universidade Federal do Paraná, Curitiba, Paraná, Brazil2

Received 26 June 2000/Returned for modification 22 August 2000/Accepted 12 September 2000

Enterococcus faecium 10/96A from Brazil was resistant to vancomycin (MIC, 256 µg/ml) but gave no amplification products with primers specific for known van genotypes. A 2,368-bp fragment of a van cluster contained one open reading frame encoding a peptide with 83% amino acid identity to VanHD, and a second encoding a D-alanine-D-lactate ligase with 83 to 85% identity to VanD. The divergent glycopeptide resistance phenotype was designated VanD4.

* Corresponding author. Mailing address: Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44-20-8200-4400, ext. 4255. Fax: 44-20-8358-3292. E-mail: nwoodford{at}phls.org.uk.

Antimicrobial Agents and Chemotherapy, December 2000, p. 3444-3446, Vol. 44, No. 12
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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