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Antimicrobial Agents and Chemotherapy, February 2000, p. 453-455, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of TEM-56, a Novel beta -Lactamase Produced by a Klebsiella pneumoniae Clinical Isolate

Catherine Neuwirth,1 Roger Labia,2,* Eliane Siebor,1 Andre Pechinot,1 Stephanie Madec,2 El Bachir Chaibi,2 and Antoine Kazmierczak1

Laboratoire de Bactériologie, Hôpital Universitaire du Bocage, B.P. 1542, 21034 Dijon Cedex,1 and Centre National de la Recherche Scientifique, UMR175, 6 rue de l'Université, 29000 Quimper,2 France

Received 10 May 1999/Returned for modification 18 August 1999/Accepted 5 November 1999

TEM-56 produced by a Klebsiella pneumoniae clinical isolate is a novel beta -lactamase of isoelectric point 6.4 that confers a moderate resistance level to expanded-spectrum cephalosporins. The amino acid sequence deduced from the corresponding bla gene showed two amino acid replacements with respect to the TEM-2 sequence: Glu-104 to Lys and His-153 to Arg. This enzyme showed catalytic properties close to those of TEM-18. Thus, TEM-56 appears as a new TEM mutant, an intermediary between TEM-18 and the extended-spectrum beta -lactamase TEM-21.

* Corresponding author. Mailing address: Centre National de la Recherche Scientifique, UMR175, 6 rue de l'Université, 29000 Quimper, France. Phone: 33-2 98 90 80 35. Fax: 33-2 98 90 80 31. E-mail: roger.labia{at}univ-brest.fr.

Antimicrobial Agents and Chemotherapy, February 2000, p. 453-455, Vol. 44, No. 2
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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