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Antimicrobial Agents and Chemotherapy, March 2000, p. 688-692, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Standardized Plaque Reduction Assay for Determination of Drug Susceptibilities of Cytomegalovirus Clinical Isolates

Marie L. Landry,1,* Sylvia Stanat,2 Karen Biron,2 Donald Brambilla,3 William Britt,4 Janet Jokela,5 Sunwen Chou,6 W. Lawrence Drew,7 Alejo Erice,8 Bruce Gilliam,9 Nell Lurain,10 Jody Manischewitz,11 Richard Miner,7 Mostafa Nokta,12 Patricia Reichelderfer,13 Stephen Spector,14 Adriana Weinberg,15 Belinda Yen-Lieberman,16 Clyde Crumpacker,5 and the AIDS Clinical Trials Group CMV Resistance Working Group

Yale University, New Haven, Connecticut1; Glaxo Wellcome, Research Triangle Park, North Carolina2; New England Research Institute, Watertown, Massachusetts3; University of Alabama, Birmingham, Alabama4; University of Oregon, Portland, Oregon6; University of California, San Francisco, California7; University of Minnesota, Minneapolis, Minnesota8; University of North Carolina, Chapel Hill, North Carolina9; Rush Medical College, Chicago, Illinois10; National Institutes of Health and Food and Drug Administration,11 and Division of AIDS, National Institute of Allergy and Infectious Diseases, National Institutes of Health,13 Bethesda, Maryland; University of Texas, Galveston, Texas12; University of California, San Diego, California14; University of Colorado, Denver, Colorado15; Cleveland Clinic Foundation, Cleveland, Ohio16; and Harvard University, Boston, Massachusetts5

Received 22 July 1999/Returned for modification 22 September 1999/Accepted 29 November 1999

Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cytomegalovirus (CMV) cell-associated clinical isolates. Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two isolates. Analysis of these results indicates the problems associated with clinical isolates, including the large genetic variability and the highly cell-associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu.

Antimicrobial Agents and Chemotherapy, March 2000, p. 688-692, Vol. 44, No. 3
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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