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Antimicrobial Agents and Chemotherapy, May 2000, p. 1387-1390, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Contributions of the AmpC beta -Lactamase and the AcrAB Multidrug Efflux System in Intrinsic Resistance of Escherichia coli K-12 to beta -Lactams

Annarita Mazzariol,1,2 Giuseppe Cornaglia,1 and Hiroshi Nikaido2,*

Institute of Microbiology, University of Verona, Verona, Italy,1 and Department of Molecular and Cell Biology, University of California, Berkeley, California2

Received 7 October 1999/Returned for modification 19 January 2000/Accepted 1 February 2000

The roles of the AmpC chromosomal beta -lactamase and the AcrAB efflux system in levels of intrinsic resistance and susceptibility of Escherichia coli to beta -lactams were studied with a set of isogenic strains. MICs of ureidopenicillins, carbenicillin, oxacillin, and cloxacillin were drastically reduced by the inactivation of AcrAB, whereas those of the earlier cephalosporins were affected mostly by the loss of AmpC beta -lactamase.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Room 229, Stanley Hall, University of California, Berkeley, CA 94720-3206. Phone: (510) 642-2027. Fax: (510) 643-9290. E-mail: nhiroshi{at}uclink4.berkeley.edu.

Antimicrobial Agents and Chemotherapy, May 2000, p. 1387-1390, Vol. 44, No. 5
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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