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Antimicrobial Agents and Chemotherapy, June 2000, p. 1568-1574, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antibiotic Resistance in the ECOR Collection: Integrons and Identification of a Novel aad Gene

Didier Mazel,1 Broderick Dychinco,2,dagger Vera A. Webb,2,Dagger and Julian Davies2,*

Unité de Programmation Moléculaire et Toxicologie Génétique, Institut Pasteur, 75724 Paris cedex 15, France,1 and Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada2

Received 23 September 1999/Returned for modification 17 December 1999/Accepted 13 March 2000

The 72 Escherichia coli strains of the ECOR collection were examined for resistance to 10 different antimicrobial agents including ampicillin, tetracycline, mercury, trimethoprim, and sulfonamides. Eighteen strains were resistant to at least one of the antibiotics tested, and nearly 20% (14 of 72) were resistant to two or more. Several of the resistance determinants were shown to be carried on conjugative elements. The collection was screened for the presence of the three classes of integrons and for the sul1 gene, which is generally associated with class 1 integrons. The four strains found to carry a class 1 integron also had Tn21-encoded mercury resistance. One of the integrons encoded a novel streptomycin resistance gene, aadA7, with an attC site (or 59-base element) nearly identical to the attC site associated with the qacF gene cassette found in In40 (M.-C. Ploy, P. Courvalin, and T. Lambert, Antimicrob. Agents Chemother. 42:2557-2563, 1998). The conservation of associated attC sites among unrelated resistance cassettes is similar to arrangements found in the Vibrio cholerae superintegrons (D. Mazel, B. Dychinco, V. A. Webb, and J. Davies, Science 280:605-608, 1998) and supports the hypothesis that resistance cassettes are picked up from superintegron pools and independently assembled from unrelated genes and related attC sites.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, Room 300, Wesbrook Building, 6174 University Blvd., Vancouver, British Columbia, Canada, V6T 1Z3. Phone: (604) 882-9308. Fax: (604) 822-6041. E-mail: jed{at}unixg.ubc.ca.

dagger Present address: School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029.

Dagger Present address: Lookfar Solutions Inc., Tofino, British Columbia V0R2Z0, Canada.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1568-1574, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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