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Antimicrobial Agents and Chemotherapy, June 2000, p. 1701-1704, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of a Mycobacterium smegmatis Mutant That Is Simultaneously Resistant to D-Cycloserine and Vancomycin

Marcy Peteroy,1,2 Anatoly Severin,3 Frances Zhao,1 Dalya Rosner,1 Uri Lopatin,1 Hataichanouk Scherman,4 Aimee Belanger,5 Bohdan Harvey,1 Graham F. Hatfull,5 Patrick J. Brennan,4 and Nancy D. Connell1,2,*

Department of Microbiology and Molecular Genetics1 and National Tuberculosis Center,2 Department of Medicine, UMDNJ/New Jersey Medical School, Newark, New Jersey 07103-2714; Wyeth-Ayerst Research, Department of Infectious Disease, Pearl River, New York 109653; Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-16774; and Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 152605

Received 30 August 1999/Returned for modification 2 November 1999/Accepted 6 March 2000

A mutant of Mycobacterium smegmatis has been isolated that is simultaneously resistant to both D-cycloserine (D-CS) and vancomycin. Genetic complementation with a PBP4 homolog restores sensitivity to both drugs. Resistance to D-CS and vancomycin in this mutant is most likely due to a novel mechanism involving peptidoglycan assembly at the cell surface.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, UMDNJ/New Jersey Medical School, 185 South Orange Ave., Newark, NJ 07103-2714. Phone: (973) 972-3759. Fax: (973) 972-3644. E-mail: connell{at}umdnj.edu.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1701-1704, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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  • Feng, Z., Barletta, R. G. (2003). Roles of Mycobacterium smegmatisD-Alanine:D-Alanine Ligase and D-Alanine Racemase in the Mechanisms of Action of and Resistance to the Peptidoglycan Inhibitor D-Cycloserine. Antimicrob. Agents Chemother. 47: 283-291 [Abstract] [Full Text]  
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