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Antimicrobial Agents and Chemotherapy, June 2000, p. 1725-1727, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A New SHV-Derived Extended-Spectrum beta -Lactamase (SHV-24) That Hydrolyzes Ceftazidime through a Single-Amino-Acid Substitution (D179G) in the &OHgr;-Loop

Hiroshi Kurokawa, Tetsuya Yagi, Naohiro Shibata, Keigo Shibayama, Kazunari Kamachi, and Yoshichika Arakawa*

Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo, Japan

Received 11 June 1999/Returned for modification 15 September 1999/Accepted 19 March 2000

A new SHV-derived extended-spectrum beta -lactamase (SHV-24) conferring high-level resistance to ceftazidime but not cefotaxime and cefazolin was identified in Japan. This enzyme was encoded by a transferable 150-kb plasmid from an Escherichia coli clinical isolate. The pI and Km for CAZ of this enzyme were 7.5 and 30 µM, respectively. SHV-24 was found to have a D179G substitution in the Omega -loop of the enzyme.

* Corresponding author. Mailing address: Department of Bacterial and Blood Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 500. Fax: 81-42-561-7173. E-mail address: yarakawa{at}nih.go.jp.

Antimicrobial Agents and Chemotherapy, June 2000, p. 1725-1727, Vol. 44, No. 6
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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