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Antimicrobial Agents and Chemotherapy, July 2000, p. 1911-1916, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Amphotericin B Resistance and Membrane Fluidity in Kluyveromyces lactis Strains

Mohamed Younsi,1 Elise Ramanandraibe,1 Roger Bonaly,1,* Mireille Donner,2 and Joël Coulon1

Faculté de Pharmacie, Université Henri Poincaré, Nancy 1, UMR-CNRS 7564, LCPE Biochimie Microbienne, 54001 Nancy Cedex,1 and Groupe de Recherches sur les Interactions Moléculaires, EA 2402, Université Henri Poincaré, Nancy 1, 54511 Vandoeuvre les Nancy Cedex,2 France

Received 13 July 1999/Returned for modification 30 September 1999/Accepted 17 April 2000

The membrane fluidity of reduced-amphotericin B (AmB)-sensitivity Kluyveromyces lactis mutant strain is higher than that of the wild-type K. lactis strain. After culture of the K. lactis and K. lactis mutant cells in the presence of subinhibitory doses of AmB (10 and 125 mg/liter, respectively), the plasma membranes of both yeast strains also showed a higher fluidity than did those of control cells. High membrane fluidity was associated with changes in the structural properties of the membranes. Culture of the K. lactis and K. lactis mutant cells in the presence of AmB induced changes in membrane lipid contents. In particular, phospholipid contents were increased in both strains treated with AmB, compared with their corresponding counterparts. As a result, the sterol/phospholipid ratio decreased. The relative proportion of monounsaturated fatty acids also increased after AmB treatment. The saturated fatty acid/monounsaturated fatty acid ratio decreased in K. lactis and K. lactis mutant cells treated with AmB but also in K. lactis mutant control cells compared to that in the K. lactis wild strain. These changes in lipid composition explain the higher fluidity, which could represent a process of metabolic resistance of the yeasts to AmB.

* Corresponding author. Mailing address: Université Henri Poincaré, Nancy 1, Faculté de Pharmacie, UMR-CNRS 7564, LCPE Biochimie Microbienne, 5 rue Albert Lebrun, B.P. 403, 54001 Nancy Cedex, France. Phone: 33(0)3-83-17-88-42. Fax: 33(0)3-83-17-88-79. E-mail: bonaly{at}pharma.u-nancy.fr.

Antimicrobial Agents and Chemotherapy, July 2000, p. 1911-1916, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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