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Antimicrobial Agents and Chemotherapy, July 2000, p. 1970-1973, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning and Sequence of the Gene Encoding a Novel Cefotaxime-Hydrolyzing beta -Lactamase (CTX-M-9) from Escherichia coli in Spain

Montserrat Sabaté,1 Raül Tarragó,2 Ferran Navarro,1,* Elisenda Miró,1 Clara Vergés,1 Jordi Barbé,2 and Guillermo Prats1

Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma 08025 Barcelona,1 and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra,2 Spain

Received 21 October 1999/Returned for modification 20 February 2000/Accepted 30 March 2000

A new CTX-M-type beta -lactamase (CTX-M-9) has been cloned from a clinical cefotaxime-resistant Escherichia coli strain. Despite the close identity that exists between the CTX-M-9 and Toho-2 beta -lactamases (88%), the 35 amino acids located between residues Ala-185 and Ala-219 are totally different in both enzymes. Outside of this region there are only six amino acids substitutions between both proteins.

* Corresponding author. Mailing address: Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Av. Sant Antoni Ma Claret, 167, 08025 Barcelona, Spain. Phone: 34 93 2919071. Fax: 34 93 2919070. E-mail: fnavarror{at}hsp.santpau.es.

Antimicrobial Agents and Chemotherapy, July 2000, p. 1970-1973, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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