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Antimicrobial Agents and Chemotherapy, July 2000, p. 1983-1985, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Role of Putative Loops 2 and 3 in Imipenem Passage through the Specific Porin OprD of Pseudomonas aeruginosa

Martina M. Ochs, Manjeet Bains, and Robert E. W. Hancock*

Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

Received 3 December 1999/Returned for modification 22 March 2000/Accepted 11 April 2000

Mutant proteins with eight amino acid deletions in putative surface loops 2 and 3 of the imipenem-specific porin OprD of Pseudomonas aeruginosa failed to reconstitute imipenem susceptibility in an oprD-deficient background. The loop 3 deletion prevented the ability of imipenem to inhibit KCl conductance through the OprD channel, as previously shown for a loop 2 deletion. This suggests that both loops 2 and 3 have a role in imipenem binding to the OprD channel.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, 300-1674 University Blvd., Vancouver, B.C. V6T 1Z3, Canada. Phone: (604) 822-3308. Fax: (604) 822-6041. E-mail: bob{at}cmdr.ubc.ca

Antimicrobial Agents and Chemotherapy, July 2000, p. 1983-1985, Vol. 44, No. 7
0066-4804/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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