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Antimicrobial Agents and Chemotherapy, January 2001, p. 331-334, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.331-334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Ovine Cathelicidin SMAP29 Kills Ovine Respiratory Pathogens In Vitro and in an Ovine Model of Pulmonary Infection

K. A. Brogden,1,* V. C. Kalfa,1 M. R. Ackermann,2 D. E. Palmquist,3 P. B. McCray Jr.,4 and B. F. Tack5

Respiratory Diseases of Livestock Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa 500101; Department of Veterinary Pathology, Iowa State University, Ames, Iowa 500112; Midwest Area, USDA Agricultural Research Service, Peoria, Illinois 616043; and Departments of Pediatrics4 and Microbiology,5 College of Medicine, The University of Iowa, Iowa City, Iowa 52242

Received 14 February 2000/Returned for modification 8 May 2000/Accepted 2 October 2000

Cathelicidins are antimicrobial peptides from sheep (SMAP29 and SMAP34), rabbits (CAP11 and CAP18), rodents (CRAMP), and humans (FALL39, LL37, and h/CAP18). In a broth microdilution assay against nine ovine pathogens, SMAP29, SMAP34, mouse CRAMP, CAP18, CAP1831, CAP1828, CAP1822, and CAP1821a were the most active, with MICs as low as 0.6 µg/ml. Other cathelicidins were less active. In lambs with pneumonia, 0.5 mg of SMAP29 reduced the concentration of bacteria in both bronchoalveolar lavage fluid and consolidated pulmonary tissues. Hence, the antimicrobial activity of SMAP29 suggests that it has applications in the treatment of respiratory tract infections.

* Corresponding author. Mailing address: Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, 2300 Dayton Ave., P.O. Box 70, Ames, IA 50010. Phone: (515) 663-7534. Fax: (515) 663-7458. E-mail: kbrogden{at}nadc.ars.usda.gov.

Antimicrobial Agents and Chemotherapy, January 2001, p. 331-334, Vol. 45, No. 1
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.1.331-334.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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