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Antimicrobial Agents and Chemotherapy, November 2001, p. 3189-3194, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3189-3194.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cloning and Biochemical Characterization of FOX-5, an AmpC-Type Plasmid-Encoded beta -Lactamase from a New York City Klebsiella pneumoniae Clinical Isolate

Anne Marie Queenan,*,1 Stephen Jenkins,2,dagger and Karen Bush1

The R.W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 08869,1 and The Mount Sinai Hospital, New York, New York 100292

Received 9 March 2001/Returned for modification 18 May 2001/Accepted 21 August 2001

Klebsiella pneumoniae 5064, isolated in New York, carried plasmid-mediated resistance to multiple beta -lactams and was unresponsive to clavulanic acid. The beta -lactamase gene responsible for cephalosporin resistance encoded FOX-5, with 96 to 97% amino acid identities to other members of the FOX family of beta -lactamases. The blaFOX-5 coding region was located next to a transposase gene from the Aeromonas salmonicida insertion element ISAS2.

* Corresponding author. Mailing address: The R.W. Johnson Pharmaceutical Research Institute, Raritan, NJ 08869. Phone: (908) 704-5515. Fax: (908) 707-3501. E-mail: aqueenan{at}prius.jnj.com.

dagger Present address: Aventis, Bridgewater, NJ 08807.

Antimicrobial Agents and Chemotherapy, November 2001, p. 3189-3194, Vol. 45, No. 11
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.11.3189-3194.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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