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Antimicrobial Agents and Chemotherapy, December 2001, p. 3497-3503, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3497-3503.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

SmeDEF Multidrug Efflux Pump Contributes to Intrinsic Multidrug Resistance in Stenotrophomonas maltophilia

Li Zhang, Xian-Zhi Li, and Keith Poole*

Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6

Received 9 February 2001/Returned for modification 29 May 2001/Accepted 22 September 2001

Stenotrophomonas maltophilia is an emerging nosocomial pathogen that displays high-level intrinsic resistance to a variety of structurally unrelated antimicrobial agents. Efflux mechanisms are known to contribute to acquired multidrug resistance in this organism, and indeed, one such multidrug efflux system, SmeDEF, was recently identified. Still, the importance of SmeDEF to intrinsic antibiotic resistance in S. maltophilia had not yet been determined. Reverse transcription-PCR confirmed expression of the smeDEF genes in wild-type S. maltophilia, and deletion of smeE or smeF in wild-type strains rendered the mutants hypersusceptible to several antimicrobials, suggesting that SmeDEF contributes to intrinsic antimicrobial resistance in this organism. Expression of smeDEF was also enhanced in an in vitro-selected multidrug-resistant mutant, although deletion of smeF but not of smeE in these mutants compromised antimicrobial resistance. Apparently, hyperexpressed SmeF is capable of functioning with additional multidrug efflux components to promote multidrug resistance in S. maltophilia.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Botterell Hall, Room 813, Stuart St., Kingston, Ontario K7L 3N6, Canada. Phone: (613) 533-6677. Fax: 613-533-6796. E-mail: poolek{at}post.queensu.ca.

Antimicrobial Agents and Chemotherapy, December 2001, p. 3497-3503, Vol. 45, No. 12
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.12.3497-3503.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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