This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Poirel, L.
Right arrow Articles by Nordmann, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Poirel, L.
Right arrow Articles by Nordmann, P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2001, p. 447-453, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.447-453.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

OXA-28, an Extended-Spectrum Variant of OXA-10 beta -Lactamase from Pseudomonas aeruginosa and Its Plasmid- and Integron-Located Gene

Laurent Poirel, Delphine Girlich, Thierry Naas, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre, France

Received 14 February 2000/Returned for modification 13 June 2000/Accepted 28 October 2000

Pseudomonas aeruginosa ED-1, isolated from a pulmonary brush of a patient hospitalized in a suburb of Paris, France, was resistant to ceftazidime and of intermediate susceptibility to ureidopenicillins and to cefotaxime. Cloning and expression of the beta -lactamase gene content of this isolate in Escherichia coli DH10B identified a novel OXA-10 variant, OXA-28, with a pI value of 8.1 and a molecular mass of 29 kDa. It differed from OXA-10 by 10 amino acid changes and from OXA-13 and OXA-19 by 2 amino acid changes, including a glycine instead of tryptophan at position 164, which is likely involved in its resistance to ceftazidime. Like OXA-11, -14, -16, and -19 and as opposed to OXA-17, OXA-28 predominantly compromised ceftazidime and had only marginal effect on the MICs of aztreonam and cefotaxime in P. aeruginosa. Once expressed in E. coli, OXA-28 raised the MIC of ceftazidime to a much higher level than those of amoxicillin, cephalothin, and cefotaxime (128, 16, 8, and 4 µg/ml, respectively). OXA-28 beta -lactamase had a broad spectrum of activity, including ceftazidime. Its activity was partially antagonized by clavulanic acid (50% inhibitory concentration, 10 µM) and NaCl addition. The oxa28 gene cassette was inserted in the variable region of a class 1 integron, In57, immediately downstream of an amino 6'-N-acetyltransferase gene cassette, aac(6')Ib. The structures of the integrons carrying either oxa28, oxa13, or oxa19 gene cassettes were almost identical, suggesting that they may have derived from a common ancestor as a result of the common European origin of the P. aeruginosa isolates. In57 was located on a self-transferable plasmid of ca. 150 kb that was transferred from P. aeruginosa to P. aeruginosa.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, February 2001, p. 447-453, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.447-453.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Higgins, P. G., Poirel, L., Lehmann, M., Nordmann, P., Seifert, H. (2009). OXA-143, a Novel Carbapenem-Hydrolyzing Class D {beta}-Lactamase in Acinetobacter baumannii. Antimicrob. Agents Chemother. 53: 5035-5038 [Abstract] [Full Text]  
  • Poirel, L., Mugnier, P. D., Toleman, M. A., Walsh, T. R., Rapoport, M. J., Petroni, A., Nordmann, P. (2009). ISCR2, Another Vehicle for blaVEB Gene Acquisition. Antimicrob. Agents Chemother. 53: 4940-4943 [Abstract] [Full Text]  
  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Potron, A., Poirel, L., Croize, J., Chanteperdrix, V., Nordmann, P. (2009). Genetic and Biochemical Characterization of the First Extended-Spectrum CARB-Type ss-Lactamase, RTG-4, from Acinetobacter baumannii. Antimicrob. Agents Chemother. 53: 3010-3016 [Abstract] [Full Text]  
  • Bethel, C. R., Distler, A. M., Ruszczycky, M. W., Carey, M. P., Carey, P. R., Hujer, A. M., Taracila, M., Helfand, M. S., Thomson, J. M., Kalp, M., Anderson, V. E., Leonard, D. A., Hujer, K. M., Abe, T., Venkatesan, A. M., Mansour, T. S., Bonomo, R. A. (2008). Inhibition of OXA-1 {beta}-Lactamase by Penems. Antimicrob. Agents Chemother. 52: 3135-3143 [Abstract] [Full Text]  
  • Carrer, A., Poirel, L., Eraksoy, H., Cagatay, A. A., Badur, S., Nordmann, P. (2008). Spread of OXA-48-Positive Carbapenem-Resistant Klebsiella pneumoniae Isolates in Istanbul, Turkey. Antimicrob. Agents Chemother. 52: 2950-2954 [Abstract] [Full Text]  
  • Meziane-Cherif, D., Lambert, T., Dupechez, M., Courvalin, P., Galimand, M. (2008). Genetic and Biochemical Characterization of OXA-63, a New Class D {beta}-Lactamase from Brachyspira pilosicoli BM4442. Antimicrob. Agents Chemother. 52: 1264-1268 [Abstract] [Full Text]  
  • Meziane-Cherif, D., Decre, D., Hoiby, E. A., Courvalin, P., Perichon, B. (2008). Genetic and Biochemical Characterization of CAD-1, a Chromosomally Encoded New Class A Penicillinase from Carnobacterium divergens. Antimicrob. Agents Chemother. 52: 551-556 [Abstract] [Full Text]  
  • Girlich, D., Leclercq, R., Naas, T., Nordmann, P. (2007). Molecular and Biochemical Characterization of the Chromosome-Encoded Class A {beta}-Lactamase BCL-1 from Bacillus clausii. Antimicrob. Agents Chemother. 51: 4009-4014 [Abstract] [Full Text]  
  • Poirel, L., Corvec, S., Rapoport, M., Mugnier, P., Petroni, A., Pasteran, F., Faccone, D., Galas, M., Drugeon, H., Cattoir, V., Nordmann, P. (2007). Identification of the Novel Narrow-Spectrum {beta}-Lactamase SCO-1 in Acinetobacter spp. from Argentina. Antimicrob. Agents Chemother. 51: 2179-2184 [Abstract] [Full Text]  
  • Poirel, L., Cattoir, V., Soares, A., Soussy, C.-J., Nordmann, P. (2007). Novel Ambler Class A {beta}-Lactamase LAP-1 and Its Association with the Plasmid-Mediated Quinolone Resistance Determinant QnrS1. Antimicrob. Agents Chemother. 51: 631-637 [Abstract] [Full Text]  
  • Girlich, D., Poirel, L., Schluter, A., Nordmann, P. (2005). TLA-2, a Novel Ambler Class A Expanded-Spectrum {beta}-Lactamase. Antimicrob. Agents Chemother. 49: 4767-4770 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Heritier, C., Poirel, L., Lambert, T., Nordmann, P. (2005). Contribution of Acquired Carbapenem-Hydrolyzing Oxacillinases to Carbapenem Resistance in Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 3198-3202 [Abstract] [Full Text]  
  • Poirel, L., Van De Loo, M., Mammeri, H., Nordmann, P. (2005). Association of Plasmid-Mediated Quinolone Resistance with Extended-Spectrum {beta}-Lactamase VEB-1. Antimicrob. Agents Chemother. 49: 3091-3094 [Abstract] [Full Text]  
  • Poole, K. (2005). Aminoglycoside Resistance in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 479-487 [Full Text]  
  • Poirel, L., Marque, S., Heritier, C., Segonds, C., Chabanon, G., Nordmann, P. (2005). OXA-58, a Novel Class D {beta}-Lactamase Involved in Resistance to Carbapenems in Acinetobacter baumannii. Antimicrob. Agents Chemother. 49: 202-208 [Abstract] [Full Text]  
  • Girlich, D., Naas, T., Nordmann, P. (2004). Biochemical Characterization of the Naturally Occurring Oxacillinase OXA-50 of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 48: 2043-2048 [Abstract] [Full Text]  
  • Heritier, C., Poirel, L., Nordmann, P. (2004). Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D {beta}-Lactamase from Shewanella algae. Antimicrob. Agents Chemother. 48: 1670-1675 [Abstract] [Full Text]  
  • Poirel, L., Magalhaes, M., Lopes, M., Nordmann, P. (2004). Molecular Analysis of Metallo-{beta}-Lactamase Gene blaSPM-1-Surrounding Sequences from Disseminated Pseudomonas aeruginosa Isolates in Recife, Brazil. Antimicrob. Agents Chemother. 48: 1406-1409 [Abstract] [Full Text]  
  • Poirel, L., Heritier, C., Tolun, V., Nordmann, P. (2004). Emergence of Oxacillinase-Mediated Resistance to Imipenem in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48: 15-22 [Abstract] [Full Text]  
  • Poirel, L., Heritier, C., Nordmann, P. (2004). Chromosome-Encoded Ambler Class D {beta}-Lactamase of Shewanella oneidensis as a Progenitor of Carbapenem-Hydrolyzing Oxacillinase. Antimicrob. Agents Chemother. 48: 348-351 [Abstract] [Full Text]  
  • Correia, M., Boavida, F., Grosso, F., Salgado, M. J., Lito, L. M., Cristino, J. M., Mendo, S., Duarte, A. (2003). Molecular Characterization of a New Class 3 Integron in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 47: 2838-2843 [Abstract] [Full Text]  
  • Bert, F., Ould-Hocine, Z., Juvin, M., Dubois, V., Loncle-Provot, V., Lefranc, V., Quentin, C., Lambert, N., Arlet, G. (2003). Evaluation of the Osiris Expert System for Identification of {beta}-Lactam Phenotypes in Isolates of Pseudomonas aeruginosa. J. Clin. Microbiol. 41: 3712-3718 [Abstract] [Full Text]  
  • Casin, I., Hanau-Bercot, B., Podglajen, I., Vahaboglu, H., Collatz, E. (2003). Salmonella enterica Serovar Typhimurium blaPER-1-Carrying Plasmid pSTI1 Encodes an Extended-Spectrum Aminoglycoside 6'-N-Acetyltransferase of Type Ib. Antimicrob. Agents Chemother. 47: 697-703 [Abstract] [Full Text]  
  • Heritier, C., Poirel, L., Aubert, D., Nordmann, P. (2003). Genetic and Functional Analysis of the Chromosome-Encoded Carbapenem-Hydrolyzing Oxacillinase OXA-40 of Acinetobacter baumannii. Antimicrob. Agents Chemother. 47: 268-273 [Abstract] [Full Text]  
  • Niumsup, P., Wuthiekanun, V. (2002). Cloning of the class D {beta}-lactamase gene from Burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. J Antimicrob Chemother 50: 445-455 [Abstract] [Full Text]  
  • De Champs, C., Poirel, L., Bonnet, R., Sirot, D., Chanal, C., Sirot, J., Nordmann, P. (2002). Prospective Survey of {beta}-Lactamases Produced by Ceftazidime- Resistant Pseudomonas aeruginosa Isolated in a French Hospital in 2000. Antimicrob. Agents Chemother. 46: 3031-3034 [Abstract] [Full Text]  
  • Bert, F., Branger, C., Lambert-Zechovsky, N. (2002). Identification of PSE and OXA {beta}-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J Antimicrob Chemother 50: 11-18 [Abstract] [Full Text]  
  • Dubois, V., Poirel, L., Marie, C., Arpin, C., Nordmann, P., Quentin, C. (2002). Molecular Characterization of a Novel Class 1 Integron Containing blaGES-1 and a Fused Product of aac(3)-Ib/aac(6"")-Ib"" Gene Cassettes in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 638-645 [Abstract] [Full Text]  
  • Poirel, L., Gerome, P., De Champs, C., Stephanazzi, J., Naas, T., Nordmann, P. (2002). Integron-Located oxa-32 Gene Cassette Encoding an Extended-Spectrum Variant of OXA-2 {beta}-Lactamase from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 566-569 [Abstract] [Full Text]  
  • Franceschini, N., Boschi, L., Pollini, S., Herman, R., Perilli, M., Galleni, M., Frere, J.-M., Amicosante, G., Rossolini, G. M. (2001). Characterization of OXA-29 from Legionella (Fluoribacter) gormanii: Molecular Class D beta -Lactamase with Unusual Properties. Antimicrob. Agents Chemother. 45: 3509-3516 [Abstract] [Full Text]  
  • Aubert, D., Poirel, L., Ben Ali, A., Goldstein, F. W., Nordmann, P. (2001). OXA-35 is an OXA-10-related {beta}-lactamase from Pseudomonas aeruginosa. J Antimicrob Chemother 48: 717-721 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Steward, C. D., Rasheed, J. K., Hubert, S. K., Biddle, J. W., Raney, P. M., Anderson, G. J., Williams, P. P., Brittain, K. L., Oliver, A., McGowan, J. E. Jr., Tenover, F. C. (2001). Characterization of Clinical Isolates of Klebsiella pneumoniae from 19 Laboratories Using the National Committee for Clinical Laboratory Standards Extended-Spectrum {beta}-Lactamase Detection Methods. J. Clin. Microbiol. 39: 2864-2872 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»