This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pai, H.
Right arrow Articles by Gotoh, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pai, H.
Right arrow Articles by Gotoh, N.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2001, p. 480-484, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.480-484.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Carbapenem Resistance Mechanisms in Pseudomonas aeruginosa Clinical Isolates

Hyunjoo Pai,1,* Jong-Won Kim,2 Jungmin Kim,3 Ji Hyang Lee,1 Kang Won Choe,4 and Naomasa Gotoh5

Division of Infectious Disease, Department of Internal Medicine,1 and Department of Microbiology,3 College of Medicine, University of Dankook, Chonan, Department of Clinical Pathology, Sungkyunkwan University College of Medicine,2 and Department of Internal Medicine, Seoul National University College of Medicine,4 Seoul, Korea, and Department of Microbiology, Kyoto Pharmaceutical University, Kyoto, Japan5

Received 31 March 2000/Returned for modification 8 August 2000/Accepted 10 November 2000

In order to define the contributions of the mechanisms for carbapenem resistance in clinical strains of Pseudomonas aeruginosa, we investigated the presence of OprD, the expressions of the MexAB-OprM and MexEF-OprN systems, and the production of the beta -lactamases for 44 clinical strains. All of the carbapenem-resistant isolates showed the loss of or decreased levels of OprD. Three strains overexpressed the MexAB-OprM efflux system by carrying mutations in mexR. These three strains had the amino acid substitution in MexR protein, Arg (CGG) right-arrow Gln (CAG), at the position of amino acid 70. None of the isolates, however, expressed the MexEF-OprN efflux system. For the characterization of beta -lactamases, at least 13 isolates were the depressed mutants, and 12 strains produced secondary beta -lactamases. Based on the above resistance mechanisms, the MICs of carbapenem for the isolates were analyzed. The MICs of carbapenem were mostly determined by the expression of OprD. The MICs of meropenem were two- to four-fold increased for the isolates which overexpressed MexAB-OprM in the background of OprD loss. However, the elevated MICs of meropenem for some individual isolates could not be explained. These findings suggested that other resistance mechanisms would play a role in meropenem resistance in clinical isolates of P. aeruginosa.

* Corresponding author. Mailing address: Division of Infectious Disease, Department of Internal Medicine, College of Medicine, University of Dankook, San 29, Anseo-dong, Chonan, Chungnam, 330-715, Korea. Phone: 82-41-550-3918. Fax: 82-41-556-3256. E-mail: paihj{at}unitel.co.kr.

Antimicrobial Agents and Chemotherapy, February 2001, p. 480-484, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.480-484.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lister, P. D., Wolter, D. J., Hanson, N. D. (2009). Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms. Clin. Microbiol. Rev. 22: 582-610 [Abstract] [Full Text]  
  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Pena, C., Suarez, C., Tubau, F., Juan, C., Moya, B., Dominguez, M. A., Oliver, A., Pujol, M., Ariza, J. (2009). Nosocomial Outbreak of a Non-Cefepime-Susceptible Ceftazidime-Susceptible Pseudomonas aeruginosa Strain Overexpressing MexXY-OprM and Producing an Integron-Borne PSE-1 ss-Lactamase. J. Clin. Microbiol. 47: 2381-2387 [Abstract] [Full Text]  
  • Zhao, W.-H., Chen, G., Ito, R., Hu, Z.-Q. (2009). Relevance of resistance levels to carbapenems and integron-borne blaIMP-1, blaIMP-7, blaIMP-10 and blaVIM-2 in clinical isolates of Pseudomonas aeruginosa. J Med Microbiol 58: 1080-1085 [Abstract] [Full Text]  
  • Siarkou, V. I., Vitti, D., Protonotariou, E., Ikonomidis, A., Sofianou, D. (2009). Molecular Epidemiology of Outbreak-Related Pseudomonas aeruginosa Strains Carrying the Novel Variant blaVIM-17 Metallo-{beta}-Lactamase Gene. Antimicrob. Agents Chemother. 53: 1325-1330 [Abstract] [Full Text]  
  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Tanimoto, K., Tomita, H., Fujimoto, S., Okuzumi, K., Ike, Y. (2008). Fluoroquinolone Enhances the Mutation Frequency for Meropenem-Selected Carbapenem Resistance in Pseudomonas aeruginosa, but Use of the High-Potency Drug Doripenem Inhibits Mutant Formation. Antimicrob. Agents Chemother. 52: 3795-3800 [Abstract] [Full Text]  
  • Koga, T., Masuda, N., Kakuta, M., Namba, E., Sugihara, C., Fukuoka, T. (2008). Potent In Vitro Activity of Tomopenem (CS-023) against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 52: 2849-2854 [Abstract] [Full Text]  
  • Gad, G. F., El-Domany, R. A., Zaki, S., Ashour, H. M. (2007). Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms. J Antimicrob Chemother 60: 1010-1017 [Abstract] [Full Text]  
  • Henrichfreise, B., Wiegand, I., Pfister, W., Wiedemann, B. (2007). Resistance Mechanisms of Multiresistant Pseudomonas aeruginosa Strains from Germany and Correlation with Hypermutation. Antimicrob. Agents Chemother. 51: 4062-4070 [Abstract] [Full Text]  
  • Henrichfreise, B., Wiegand, I., Luhmer-Becker, I., Wiedemann, B. (2007). Development of Resistance in Wild-Type and Hypermutable Pseudomonas aeruginosa Strains Exposed to Clinical Pharmacokinetic Profiles of Meropenem and Ceftazidime Simulated In Vitro. Antimicrob. Agents Chemother. 51: 3642-3649 [Abstract] [Full Text]  
  • Walther-Rasmussen, J., Hoiby, N. (2007). Class A carbapenemases. J Antimicrob Chemother 60: 470-482 [Abstract] [Full Text]  
  • Kwon, D.-H., Lu, C.-D. (2007). Polyamine Effects on Antibiotic Susceptibility in Bacteria. Antimicrob. Agents Chemother. 51: 2070-2077 [Abstract] [Full Text]  
  • Kaye, K. S., Kanafani, Z. A., Dodds, A. E., Engemann, J. J., Weber, S. G., Carmeli, Y. (2006). Differential Effects of Levofloxacin and Ciprofloxacin on the Risk for Isolation of Quinolone-Resistant Pseudomonas aeruginosa.. Antimicrob. Agents Chemother. 50: 2192-2196 [Abstract] [Full Text]  
  • Quale, J., Bratu, S., Gupta, J., Landman, D. (2006). Interplay of Efflux System, ampC, and oprD Expression in Carbapenem Resistance of Pseudomonas aeruginosa Clinical Isolates.. Antimicrob. Agents Chemother. 50: 1633-1641 [Abstract] [Full Text]  
  • Walther-Rasmussen, J., Hoiby, N. (2006). OXA-type carbapenemases. J Antimicrob Chemother 57: 373-383 [Abstract] [Full Text]  
  • Salunkhe, P., Smart, C. H. M., Morgan, J. A. W., Panagea, S., Walshaw, M. J., Hart, C. A., Geffers, R., Tummler, B., Winstanley, C. (2005). A Cystic Fibrosis Epidemic Strain of Pseudomonas aeruginosa Displays Enhanced Virulence and Antimicrobial Resistance. J. Bacteriol. 187: 4908-4920 [Abstract] [Full Text]  
  • Poole, K. (2005). Efflux-mediated antimicrobial resistance. J Antimicrob Chemother 56: 20-51 [Abstract] [Full Text]  
  • Bratu, S., Tolaney, P., Karumudi, U., Quale, J., Mooty, M., Nichani, S., Landman, D. (2005). Carbapenemase-producing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents. J Antimicrob Chemother 56: 128-132 [Abstract] [Full Text]  
  • Quinteira, S., Sousa, J. C., Peixe, L. (2005). Characterization of In100, a New Integron Carrying a Metallo-{beta}-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 451-453 [Abstract] [Full Text]  
  • Crespo, M. P., Woodford, N., Sinclair, A., Kaufmann, M. E., Turton, J, Glover, J., Velez, J. D., Castaneda, C. R., Recalde, M., Livermore, D. M. (2004). Outbreak of Carbapenem-Resistant Pseudomonas aeruginosa Producing VIM-8, a Novel Metallo-{beta}-Lactamase, in a Tertiary Care Center in Cali, Colombia. J. Clin. Microbiol. 42: 5094-5101 [Abstract] [Full Text]  
  • Llanes, C., Hocquet, D., Vogne, C., Benali-Baitich, D., Neuwirth, C., Plesiat, P. (2004). Clinical Strains of Pseudomonas aeruginosa Overproducing MexAB-OprM and MexXY Efflux Pumps Simultaneously. Antimicrob. Agents Chemother. 48: 1797-1802 [Abstract] [Full Text]  
  • Yomoda, S., Okubo, T., Takahashi, A., Murakami, M., Iyobe, S. (2003). Presence of Pseudomonas putida Strains Harboring Plasmids Bearing the Metallo-{beta}-Lactamase Gene blaIMP in a Hospital in Japan. J. Clin. Microbiol. 41: 4246-4251 [Abstract] [Full Text]  
  • Bert, F., Ould-Hocine, Z., Juvin, M., Dubois, V., Loncle-Provot, V., Lefranc, V., Quentin, C., Lambert, N., Arlet, G. (2003). Evaluation of the Osiris Expert System for Identification of {beta}-Lactam Phenotypes in Isolates of Pseudomonas aeruginosa. J. Clin. Microbiol. 41: 3712-3718 [Abstract] [Full Text]  
  • Hocquet, D., Bertrand, X., Kohler, T., Talon, D., Plesiat, P. (2003). Genetic and Phenotypic Variations of a Resistant Pseudomonas aeruginosa Epidemic Clone. Antimicrob. Agents Chemother. 47: 1887-1894 [Abstract] [Full Text]  
  • Kim, Y.-K., Pai, H., Lee, H.-J., Park, S.-E., Choi, E.-H., Kim, J., Kim, J.-H., Kim, E.-C. (2002). Bloodstream Infections by Extended-Spectrum {beta}-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Children: Epidemiology and Clinical Outcome. Antimicrob. Agents Chemother. 46: 1481-1491 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»