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Antimicrobial Agents and Chemotherapy, February 2001, p. 605-607, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.605-607.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Germinated and Nongerminated Conidial Suspensions for Testing of Susceptibilities of Aspergillus spp. to Amphotericin B, Itraconazole, Posaconazole, Ravuconazole, and Voriconazole

A. Espinel-Ingroff*

Medical College of Virginia/Virginia Commonwealth University, Richmond, Virginia 23298

Received 14 August 2000/Returned for modification 5 October 2000/Accepted 20 November 2000

The effect of germinated and nongerminated conidia of Aspergillus spp. on the fungistatic (National Committee for Clinical Laboratory Standards document M38-P) and fungicidal activities (MICs and minimal fungicidal concentrations [MFCs] respectively) of amphotericin B, itraconazole, posaconazole (SCH56592), ravuconazole (BMS-207147), and voriconazole was evaluated. MFCs were the lowest drug dilutions that showed fewer than three colonies (99.9% killing). Overall, the MICs (0.12 to 4 µg/ml) and MFCs (0.5 to >8 µg/ml) of all of the agents tested with both inocula were the same or within 2 dilutions for the 72 isolates. Therefore, MICs and MFCs can be obtained with convenient and standardized nongerminated conidia.

* Mailing address: Division of Infectious Diseases, 1101 East Marshall St., Sanger Hall Room 7049, Richmond, VA 23298. Phone: (804) 8289711. Fax: (804) 8283097. E-mail: AVINGROFF{at}HSC.VCU.EDU.

Antimicrobial Agents and Chemotherapy, February 2001, p. 605-607, Vol. 45, No. 2
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.2.605-607.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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