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Antimicrobial Agents and Chemotherapy, March 2001, p. 710-714, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.710-714.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

IMP-4, a Novel Metallo-beta -Lactamase from Nosocomial Acinetobacter spp. Collected in Hong Kong between 1994 and 1998

Yiu-Wai Chu,1 Mariya Afzal-Shah,2 Elizabeth T. S. Houang,1,* Marie-France I. Palepou,2 Donald J. Lyon,1 Neil Woodford,2 and David M. Livermore2

Department of Microbiology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China,1 and Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom2

Received 16 May 2000/Returned for modification 15 August 2000/Accepted 24 November 2000

Between 1994 and 1998, 97 imipenem-resistant Acinetobacter isolates were identified at the Prince of Wales Hospital, Hong Kong, China. A blaIMP PCR product was obtained from 23 of 35 viable cultures; 12 isolates belonged to genomic DNA group 3, 8 belonged to group 2 (Acinetobacter baumannii), 2 belonged to group 13TU, and 1 belonged to group 1. The blaIMP homologues were sequenced from two isolates from genomic DNA group 2 and one isolate each from groups 3 and 13TU. The four sequences included an identical 738-bp open reading frame, predicted to encode a polypeptide of 246 amino acids, with 95.6% homology to IMP-1 and 89.3% homology to IMP-2. The new enzyme, designated IMP-4, was partially purified. It had a pI of 8.0 and was strongly active against imipenem and meropenem, with Vmax values 53 and 8% of that for penicillin G, respectively. Strong activity was also seen against oxyimino-aminothiazolyl cephalosporins but not against aztreonam. Hydrolytic activity was inhibited by EDTA but not by clavulanate or tazobactam. Carbapenem MICs for most blaIMP-positive isolates were 4 to 32 µg/ml, but one isolate with the intact gene was susceptible, with imipenem and meropenem MICs of 0.25 and 0.5 µg/ml, respectively. The latter isolate did not produce the band with a pI of 8.0, and gene expression was inferred to have been lost. None of the isolates studied in detail contained extrachromosomal DNA, and carbapenem resistance was not transmissible to Escherichia coli. Nevertheless, the presence of blaIMP-4 in different genomic DNA groups implies horizontal transfer, and sequences resembling a GTTRRRY integrase-dependent recombination motif were identified in the flanking regions of blaIMP-4.

* Corresponding author. Mailing address: Department of Microbiology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China. Phone: 852 2632 2304. Fax: 852 2647 3227. E-mail: ehouang{at}cuhk.edu.hk.

Antimicrobial Agents and Chemotherapy, March 2001, p. 710-714, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.710-714.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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