This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goldstein, C.
Right arrow Articles by Maurer, J. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldstein, C.
Right arrow Articles by Maurer, J. J.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, March 2001, p. 723-726, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.723-726.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Incidence of Class 1 and 2 Integrases in Clinical and Commensal Bacteria from Livestock, Companion Animals, and Exotics

Cathy Goldstein,1 Margie D. Lee,1 Susan Sanchez,1 Charlene Hudson,2 Brad Phillips,2 Brad Register,2 Michael Grady,1 Cynthia Liebert,3 Anne O. Summers,3 David G. White,4 and John J. Maurer2,*

Department of Medical Microbiology and Parasitology,1 Avian Medicine,2 and Microbiology,3 The University of Georgia, Athens, Georgia 30602, and Center for Veterinary Medicine, Food and Drug Administration, Laurel, Maryland 207084

Received 23 May 2000/Returned for modification 11 September 2000/Accepted 7 December 2000

Many pathogenic and commensal organisms are multidrug resistant due to exposure to various antibiotics. Often, this antimicrobial resistance is encoded by integrons that occur on plasmids or that are integrated into the bacterial chromosome. Integrons are commonly associated with bacterial genera in the family Enterobacteriaceae. We determined that class 1 integrases were present in approximately 46% of the isolates from the family Enterobacteriaceae; class 2 integrases were present only among Escherichia coli and Salmonella isolates. Seven percent of veterinary isolates were positive for class 3 integrase by DNA-DNA hybridization but could not be confirmed to be positive by PCR. None of the veterinary isolates possessed the class 4 integrase gene. The distribution of these integrase genes was variable within the members of the family Enterobacteriaceae when some or all integrase classes were absent from a particular genus. There was also considerable variability in the distribution of these integrases within a species, depending on the animal host. Unlike the class 1 integrases, the other integrase class, intI2, appears to be more restricted in its distribution among the members of the family Enterobacteriaceae. There is also considerable variability in the distribution of the class 1 integrases within E. coli strains isolated from different food animals. The class 1 integrases are the most widely disseminated of the four classes among the members of the family Enterobacteriaceae from both the clinical and normal flora of animals. This is the first report to closely examine the distribution of class 2 integrases in members of the family Enterobacteriaceae isolated in the United States.

* Corresponding author. Mailing address: Department of Avian Medicine, The University of Georgia, Athens, GA 30602. Phone: (706) 542-5071. Fax: (706) 542-5630. E-mail: jmaurer{at}calc.vet.uga.edu.

Antimicrobial Agents and Chemotherapy, March 2001, p. 723-726, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.723-726.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Machado, E., Coque, T. M., Canton, R., Sousa, J. C., Peixe, L. (2008). Antibiotic resistance integrons and extended-spectrum {beta}-lactamases among Enterobacteriaceae isolates recovered from chickens and swine in Portugal. J Antimicrob Chemother 62: 296-302 [Abstract] [Full Text]  
  • Gillings, M., Boucher, Y., Labbate, M., Holmes, A., Krishnan, S., Holley, M., Stokes, H. W. (2008). The Evolution of Class 1 Integrons and the Rise of Antibiotic Resistance. J. Bacteriol. 190: 5095-5100 [Abstract] [Full Text]  
  • Diaz-Mejia, J. J., Amabile-Cuevas, C. F., Rosas, I., Souza, V. (2008). An analysis of the evolutionary relationships of integron integrases, with emphasis on the prevalence of class 1 integrons in Escherichia coli isolates from clinical and environmental origins. Microbiology 154: 94-102 [Abstract] [Full Text]  
  • Van, T. T. H., Moutafis, G., Tran, L. T., Coloe, P. J. (2007). Antibiotic Resistance in Food-Borne Bacterial Contaminants in Vietnam. Appl. Environ. Microbiol. 73: 7906-7911 [Abstract] [Full Text]  
  • Moura, A., Henriques, I., Ribeiro, R., Correia, A. (2007). Prevalence and characterization of integrons from bacteria isolated from a slaughterhouse wastewater treatment plant. J Antimicrob Chemother 60: 1243-1250 [Abstract] [Full Text]  
  • Smith, J. L., Drum, D. J. V., Dai, Y., Kim, J. M., Sanchez, S., Maurer, J. J., Hofacre, C. L., Lee, M. D. (2007). Impact of Antimicrobial Usage on Antimicrobial Resistance in Commensal Escherichia coli Strains Colonizing Broiler Chickens. Appl. Environ. Microbiol. 73: 1404-1414 [Abstract] [Full Text]  
  • Rodriguez, I., Martin, M. C., Mendoza, M. C., Rodicio, M. R. (2006). Class 1 and class 2 integrons in non-prevalent serovars of Salmonella enterica: structure and association with transposons and plasmids. J Antimicrob Chemother 58: 1124-1132 [Abstract] [Full Text]  
  • Barlow, R. S., Gobius, K. S. (2006). Diverse class 2 integrons in bacteria from beef cattle sources. J Antimicrob Chemother 58: 1133-1138 [Abstract] [Full Text]  
  • Stokes, H. W., Nesbo, C. L., Holley, M., Bahl, M. I., Gillings, M. R., Boucher, Y. (2006). Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community.. J. Bacteriol. 188: 5722-5730 [Abstract] [Full Text]  
  • Solberg, O. D., Ajiboye, R. M., Riley, L. W. (2006). Origin of Class 1 and 2 Integrons and Gene Cassettes in a Population-Based Sample of Uropathogenic Escherichia coli. J. Clin. Microbiol. 44: 1347-1351 [Abstract] [Full Text]  
  • Sunde, M. (2005). Prevalence and characterization of class 1 and class 2 integrons in Escherichia coli isolated from meat and meat products of Norwegian origin. J Antimicrob Chemother 56: 1019-1024 [Abstract] [Full Text]  
  • Luo, H., Wan, K., Wang, H. H. (2005). High-Frequency Conjugation System Facilitates Biofilm Formation and pAM{beta}1 Transmission by Lactococcus lactis. Appl. Environ. Microbiol. 71: 2970-2978 [Abstract] [Full Text]  
  • Machado, E., Canton, R., Baquero, F., Galan, J.-C., Rollan, A., Peixe, L., Coque, T. M. (2005). Integron Content of Extended-Spectrum-{beta}-Lactamase-Producing Escherichia coli Strains over 12 Years in a Single Hospital in Madrid, Spain. Antimicrob. Agents Chemother. 49: 1823-1829 [Abstract] [Full Text]  
  • Kang, H. Y., Jeong, Y. S., Oh, J. Y., Tae, S. H., Choi, C. H., Moon, D. C., Lee, W. K., Lee, Y. C., Seol, S. Y., Cho, D. T., Lee, J. C. (2005). Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. J Antimicrob Chemother 55: 639-644 [Abstract] [Full Text]  
  • Ahmed, A. M., Nakano, H., Shimamoto, T. (2005). Molecular characterization of integrons in non-typhoid Salmonella serovars isolated in Japan: description of an unusual class 2 integron. J Antimicrob Chemother 55: 371-374 [Abstract] [Full Text]  
  • Antunes, P., Machado, J., Sousa, J. C., Peixe, L. (2005). Dissemination of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Portuguese Salmonella enterica Strains and Relation with Integrons. Antimicrob. Agents Chemother. 49: 836-839 [Abstract] [Full Text]  
  • Nandi, S., Maurer, J. J., Hofacre, C., Summers, A. O. (2004). Gram-positive bacteria are a major reservoir of Class 1 antibiotic resistance integrons in poultry litter. Proc. Natl. Acad. Sci. USA 101: 7118-7122 [Abstract] [Full Text]  
  • Barlow, R. S., Pemberton, J. M., Desmarchelier, P. M., Gobius, K. S. (2004). Isolation and Characterization of Integron-Containing Bacteria without Antibiotic Selection. Antimicrob. Agents Chemother. 48: 838-842 [Abstract] [Full Text]  
  • Yu, H. S., Lee, J. C., Kang, H. Y., Jeong, Y. S., Lee, E. Y., Choi, C. H., Tae, S. H., Lee, Y. C., Seol, S. Y., Cho, D. T. (2004). Prevalence of dfr genes associated with integrons and dissemination of dfrA17 among urinary isolates of Escherichia coli in Korea. J Antimicrob Chemother 53: 445-450 [Abstract] [Full Text]  
  • Miko, A., Pries, K., Schroeter, A., Helmuth, R. (2003). Multiple-Drug Resistance in D-Tartrate-Positive Salmonella enterica Serovar Paratyphi B Isolates from Poultry Is Mediated by Class 2 Integrons Inserted into the Bacterial Chromosome. Antimicrob. Agents Chemother. 47: 3640-3643 [Abstract] [Full Text]  
  • Correia, M., Boavida, F., Grosso, F., Salgado, M. J., Lito, L. M., Cristino, J. M., Mendo, S., Duarte, A. (2003). Molecular Characterization of a New Class 3 Integron in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 47: 2838-2843 [Abstract] [Full Text]  
  • Mc Dermott, P. F., Walker, R. D., White, D. G. (2003). Antimicrobials: Modes of Action and Mechanisms of Resistance. International Journal of Toxicology 22: 135-143 [Abstract]  
  • Lu, J., Sanchez, S., Hofacre, C., Maurer, J. J., Harmon, B. G., Lee, M. D. (2003). Evaluation of Broiler Litter with Reference to the Microbial Composition as Assessed by Using 16S rRNA and Functional Gene Markers. Appl. Environ. Microbiol. 69: 901-908 [Abstract] [Full Text]  
  • Soto, S. M., Lobato, M. J., Mendoza, M. C. (2003). Class 1 Integron-Borne Gene Cassettes in Multidrug-Resistant Yersinia enterocolitica Strains of Different Phenotypic and Genetic Types. Antimicrob. Agents Chemother. 47: 421-426 [Abstract] [Full Text]  
  • Lee, M. D., Sanchez, S., Zimmer, M., Idris, U., Berrang, M. E., McDermott, P. F. (2002). Class 1 Integron-Associated Tobramycin-Gentamicin Resistance in Campylobacter jejuni Isolated from the Broiler Chicken House Environment. Antimicrob. Agents Chemother. 46: 3660-3664 [Abstract] [Full Text]  
  • Sanchez, S., McCrackin Stevenson, M. A., Hudson, C. R., Maier, M., Buffington, T., Dam, Q., Maurer, J. J. (2002). Characterization of Multidrug-Resistant Escherichia coli Isolates Associated with Nosocomial Infections in Dogs. J. Clin. Microbiol. 40: 3586-3595 [Abstract] [Full Text]  
  • Guerra, B., Soto, S., Helmuth, R., Mendoza, M. C. (2002). Characterization of a Self-Transferable Plasmid from Salmonella enterica Serotype Typhimurium Clinical Isolates Carrying Two Integron-Borne Gene Cassettes Together with Virulence and Drug Resistance Genes. Antimicrob. Agents Chemother. 46: 2977-2981 [Abstract] [Full Text]  
  • Bischoff, K. M., White, D. G., McDermott, P. F., Zhao, S., Gaines, S., Maurer, J. J., Nisbet, D. J. (2002). Characterization of Chloramphenicol Resistance in Beta-Hemolytic Escherichia coli Associated with Diarrhea in Neonatal Swine. J. Clin. Microbiol. 40: 389-394 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»