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Antimicrobial Agents and Chemotherapy, March 2001, p. 878-882, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.878-882.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular and Biochemical Analysis of AST-1, a Class A beta -Lactamase from Nocardia asteroides Sensu Stricto

Laurent Poirel,1 Frederic Laurent,1,2,3 Thierry Naas,1 Roger Labia,4 Patrick Boiron,3 and Patrice Nordmann1,*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex,1 Service de Bactériologie, Hôpital Lyon-Sud, Hospices Civils de Lyon, Faculté de Médecine Lyon-Sud, 69921 Oullins Cedex,2 Laboratoire de mycologie fondamentale et appliquée aux biotechnologies industrielles, Institut des Sciences Pharmaceutiques et Biologiques, 69373 Lyon Cedex 08,3 and UMR175 CNRS Chimie et Biologie des Substances Actives, 29000 Quimper,4 France

Received 22 June 2000/Returned for modification 1 October 2000/Accepted 15 December 2000

A beta -lactamase gene was cloned from a Nocardia asteroides sensu stricto clinical isolate. A recombinant plasmid, pAST-1, expressed the beta -lactamase AST-1 in Escherichia coli JM109. Its pI was 4.8, and its relative molecular mass was 31 kDa. E. coli JM109(pAST-1) was resistant to penicillins and narrow-spectrum cephalosporins. The beta -lactamase AST-1 had a restricted hydrolytic activity spectrum. Its activity was partially inhibited by clavulanic acid but not by sulbactam and tazobactam. AST-1 is an Ambler class A beta -lactamase sharing 65% amino acid identity with beta -lactamase FAR-1, the most closely related enzyme.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, March 2001, p. 878-882, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.878-882.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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