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Antimicrobial Agents and Chemotherapy, March 2001, p. 952-955, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.952-955.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Mutation in ParE in a Pneumococcal In Vitro Mutant Resistant to Fluoroquinolones

Claire Janoir,1 Emmanuelle Varon,1 Marie-Dominique Kitzis,2 and Laurent Gutmann1,*

L.R.M.A., Université Paris VI, 75270 Paris Cedex 06,1 and Service de Bactériologie, Hôpital Saint-Joseph, 75674 Paris Cedex 14,2 France

Received 27 July 2000/Returned for modification 22 September 2000/Accepted 19 December 2000

For an in vitro mutant of Streptococcus pneumoniae selected on moxifloxacin four- to eightfold-increased MICs of new fluoroquinolones, only a twofold-increased MIC of ciprofloxacin, and a twofold-decreased MIC of novobiocin were observed. This phenotype was conferred by two mutations: Ser81Phe in GyrA and a novel undescribed His103Tyr mutation in ParE, outside the quinolone resistance-determining region, in the putative ATP-binding site of topoisomerase IV.

* Corresponding author. Mailing address: L.R.M.A., Université Paris VI, 15, rue de l'Ecole de Médecine, 75270 Paris Cedex 06, France. Phone: 33-1-42.34.28.63. Fax: 33-1-43.25.68.12. E-mail: gutmann{at}ccr.jussieu.fr.

Antimicrobial Agents and Chemotherapy, March 2001, p. 952-955, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.952-955.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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