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Antimicrobial Agents and Chemotherapy, March 2001, p. 962-965, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.962-965.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mutations of the Helicobacter pylori Genes rdxA and pbp1 Cause Resistance against Metronidazole and Amoxicillin

Ralf Paul,*,1 Stefan Postius,2 Klaus Melchers,2 and Klaus P. Schäfer2

Yale University, New Haven, Connecticut 06511,1 and Byk Gulden Pharmaceuticals, Konstanz, Germany2

Received 9 June 2000/Returned for modification 17 August 2000/Accepted 25 November 2000

To investigate amoxicillin and metronidazole resistance of Helicobacter pylori, we compared putative resistance genes between resistant strains obtained in vitro and their sensitive parent strain. All metronidazole-resistant strains had rdxA mutations, and an amoxicillin-resistant strain had pbp1 and pbp2 mutations. By transforming PCR products of these mutated genes into antibiotic-sensitive strains, we showed that rdxA null mutations were sufficient for metronidazole resistance, while pbp1 mutations contributed to amoxicillin resistance of H. pylori.

* Corresponding author. Mailing address: Yale University, 266 Whitney Ave., New Haven, CT 06511. Phone: (203) 432-3506. Fax: (203) 432-5713. E-mail: Ralf.Paul{at}Yale.edu.

Antimicrobial Agents and Chemotherapy, March 2001, p. 962-965, Vol. 45, No. 3
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.3.962-965.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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