This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yigit, H.
Right arrow Articles by Tenover, F. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yigit, H.
Right arrow Articles by Tenover, F. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2001, p. 1151-1161, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1151-1161.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Carbapenem-Hydrolyzing beta -Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae

Hesna Yigit,1 Anne Marie Queenan,2 Gregory J. Anderson,1 Antonio Domenech-Sanchez,3 James W. Biddle,1 Christine D. Steward,1 Sebastian Alberti,4 Karen Bush,2 and Fred C. Tenover1,*

Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303331; The R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 088692; and Unidad de Investigacion, Hospital Son Dureta, Andrea Doria, Palma de Mallorca, 07014,4 and Área de Microbiologia, Universidad de las Islas Baleares, Crtra. Valldemosa, Palma de Mallorca, 07071,3 Spain

Received 19 September 2000/Returned for modification 21 November 2000/Accepted 23 January 2001

A Klebsiella pneumoniae isolate showing moderate to high-level imipenem and meropenem resistance was investigated. The MICs of both drugs were 16 µg/ml. The beta -lactamase activity against imipenem and meropenem was inhibited in the presence of clavulanic acid. The strain was also resistant to extended-spectrum cephalosporins and aztreonam. Isoelectric focusing studies demonstrated three beta -lactamases, with pIs of 7.2 (SHV-29), 6.7 (KPC-1), and 5.4 (TEM-1). The presence of blaSHV and blaTEM genes was confirmed by specific PCRs and DNA sequence analysis. Transformation and conjugation studies with Escherichia coli showed that the beta -lactamase with a pI of 6.7, KPC-1 (K. pneumoniae carbapenemase-1), was encoded on an approximately 50-kb nonconjugative plasmid. The gene, blaKPC-1, was cloned in E. coli and shown to confer resistance to imipenem, meropenem, extended-spectrum cephalosporins, and aztreonam. The amino acid sequence of the novel carbapenem-hydrolyzing beta -lactamase, KPC-1, showed 45% identity to the pI 9.7 carbapenem-hydrolyzing beta -lactamase, Sme-1, from Serratia marcescens S6. Hydrolysis studies showed that purified KPC-1 hydrolyzed not only carbapenems but also penicillins, cephalosporins, and monobactams. KPC-1 had the highest affinity for meropenem. The kinetic studies also revealed that clavulanic acid and tazobactam inhibited KPC-1. An examination of the outer membrane proteins of the parent K. pneumoniae strain demonstrated that the strain does not express detectable levels of OmpK35 and OmpK37, although OmpK36 is present. We concluded that carbapenem resistance in K. pneumoniae strain 1534 is mainly due to production of a novel Bush group 2f, class A, carbapenem-hydrolyzing beta -lactamase, KPC-1, although alterations in porin expression may also play a role.

* Corresponding author. Mailing address: Nosocomial Pathogens Laboratory Branch (G08), Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3375. Fax: (404) 639-1381. E-mail: fnt1{at}cdc.gov.

Antimicrobial Agents and Chemotherapy, April 2001, p. 1151-1161, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1151-1161.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Endimiani, A., DePasquale, J. M., Forero, S., Perez, F., Hujer, A. M., Roberts-Pollack, D., Fiorella, P. D., Pickens, N., Kitchel, B., Casiano-Colon, A. E., Tenover, F. C., Bonomo, R. A. (2009). Emergence of blaKPC-containing Klebsiella pneumoniae in a long-term acute care hospital: a new challenge to our healthcare system. J Antimicrob Chemother 64: 1102-1110 [Abstract] [Full Text]  
  • Tsakris, A., Poulou, A., Themeli-Digalaki, K., Voulgari, E., Pittaras, T., Sofianou, D., Pournaras, S., Petropoulou, D. (2009). Use of Boronic Acid Disk Tests To Detect Extended- Spectrum {beta}-Lactamases in Clinical Isolates of KPC Carbapenemase-Possessing Enterobacteriaceae. J. Clin. Microbiol. 47: 3420-3426 [Abstract] [Full Text]  
  • Baraniak, A., Izdebski, R., Herda, M., Fiett, J., Hryniewicz, W., Gniadkowski, M., Kern-Zdanowicz, I., Filczak, K., Lopaciuk, U. (2009). Emergence of Klebsiella pneumoniae ST258 with KPC-2 in Poland. Antimicrob. Agents Chemother. 53: 4565-4567 [Full Text]  
  • Shen, P., Wei, Z., Jiang, Y., Du, X., Ji, S., Yu, Y., Li, L. (2009). Novel Genetic Environment of the Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2 among Enterobacteriaceae in China. Antimicrob. Agents Chemother. 53: 4333-4338 [Abstract] [Full Text]  
  • Landman, D., Bratu, S., Quale, J. (2009). Contribution of OmpK36 to carbapenem susceptibility in KPC-producing Klebsiella pneumoniae. J Med Microbiol 58: 1303-1308 [Abstract] [Full Text]  
  • Hawkey, P. M., Jones, A. M. (2009). The changing epidemiology of resistance. J Antimicrob Chemother 64: i3-i10 [Abstract] [Full Text]  
  • Wang, X. D., Cai, J. C., Zhou, H. W., Zhang, R., Chen, G.-X. (2009). Reduced susceptibility to carbapenems in Klebsiella pneumoniae clinical isolates associated with plasmid-mediated {beta}-lactamase production and OmpK36 porin deficiency. J Med Microbiol 58: 1196-1202 [Abstract] [Full Text]  
  • Stachyra, T., Levasseur, P., Pechereau, M.-C., Girard, A.-M., Claudon, M., Miossec, C., Black, M. T. (2009). In vitro activity of the {beta}-lactamase inhibitor NXL104 against KPC-2 carbapenemase and Enterobacteriaceae expressing KPC carbapenemases. J Antimicrob Chemother 64: 326-329 [Abstract] [Full Text]  
  • Akpaka, P. E., Swanston, W. H., Ihemere, H. N., Correa, A., Torres, J. A., Tafur, J. D., Montealegre, M. C., Quinn, J. P., Villegas, M. V. (2009). Emergence of KPC-Producing Pseudomonas aeruginosa in Trinidad and Tobago. J. Clin. Microbiol. 47: 2670-2671 [Abstract] [Full Text]  
  • Kitchel, B., Rasheed, J. K., Patel, J. B., Srinivasan, A., Navon-Venezia, S., Carmeli, Y., Brolund, A., Giske, C. G. (2009). Molecular Epidemiology of KPC-Producing Klebsiella pneumoniae Isolates in the United States: Clonal Expansion of Multilocus Sequence Type 258. Antimicrob. Agents Chemother. 53: 3365-3370 [Abstract] [Full Text]  
  • Grobner, S., Linke, D., Schutz, W., Fladerer, C., Madlung, J., Autenrieth, I. B., Witte, W., Pfeifer, Y. (2009). Emergence of carbapenem-non-susceptible extended-spectrum {beta}-lactamase-producing Klebsiella pneumoniae isolates at the university hospital of Tubingen, Germany. J Med Microbiol 58: 912-922 [Abstract] [Full Text]  
  • Goldfarb, D., Harvey, S.-B., Jessamine, K., Jessamine, P., Toye, B., Desjardins, M. (2009). Detection of Plasmid-Mediated KPC-Producing Klebsiella pneumoniae in Ottawa, Canada: Evidence of Intrahospital Transmission. J. Clin. Microbiol. 47: 1920-1922 [Abstract] [Full Text]  
  • Gootz, T. D., Lescoe, M. K., Dib-Hajj, F., Dougherty, B. A., He, W., Della-Latta, P., Huard, R. C. (2009). Genetic Organization of Transposase Regions Surrounding blaKPC Carbapenemase Genes on Plasmids from Klebsiella Strains Isolated in a New York City Hospital. Antimicrob. Agents Chemother. 53: 1998-2004 [Abstract] [Full Text]  
  • Lee, J., Patel, G., Huprikar, S., Calfee, D. P., Jenkins, S. G. (2009). Decreased Susceptibility to Polymyxin B during Treatment for Carbapenem-Resistant Klebsiella pneumoniae Infection. J. Clin. Microbiol. 47: 1611-1612 [Full Text]  
  • Leavitt, A., Chmelnitsky, I., Colodner, R., Ofek, I., Carmeli, Y., Navon-Venezia, S. (2009). Ertapenem Resistance among Extended-Spectrum-{beta}-Lactamase-Producing Klebsiella pneumoniae Isolates. J. Clin. Microbiol. 47: 969-974 [Abstract] [Full Text]  
  • Samuelsen, O., Naseer, U., Tofteland, S., Skutlaberg, D. H., Onken, A., Hjetland, R., Sundsfjord, A., Giske, C. G. (2009). Emergence of clonally related Klebsiella pneumoniae isolates of sequence type 258 producing plasmid-mediated KPC carbapenemase in Norway and Sweden. J Antimicrob Chemother 63: 654-658 [Abstract] [Full Text]  
  • Doumith, M., Ellington, M. J., Livermore, D. M., Woodford, N. (2009). Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J Antimicrob Chemother 63: 659-667 [Abstract] [Full Text]  
  • McGettigan, S. E., Andreacchio, K., Edelstein, P. H. (2009). Specificity of Ertapenem Susceptibility Screening for Detection of Klebsiella pneumoniae Carbapenemases. J. Clin. Microbiol. 47: 785-786 [Abstract] [Full Text]  
  • Endimiani, A., Hujer, A. M., Perez, F., Bethel, C. R., Hujer, K. M., Kroeger, J., Oethinger, M., Paterson, D. L., Adams, M. D., Jacobs, M. R., Diekema, D. J., Hall, G. S., Jenkins, S. G., Rice, L. B., Tenover, F. C., Bonomo, R. A. (2009). Characterization of blaKPC-containing Klebsiella pneumoniae isolates detected in different institutions in the Eastern USA. J Antimicrob Chemother 63: 427-437 [Abstract] [Full Text]  
  • Wolter, D. J., Kurpiel, P. M., Woodford, N., Palepou, M.-F. I., Goering, R. V., Hanson, N. D. (2009). Phenotypic and Enzymatic Comparative Analysis of the Novel KPC Variant KPC-5 and Its Evolutionary Variants, KPC-2 and KPC-4. Antimicrob. Agents Chemother. 53: 557-562 [Abstract] [Full Text]  
  • Peirano, G., Seki, L. M., Val Passos, V. L., Pinto, M. C. F. G., Guerra, L. R., Asensi, M. D. (2009). Carbapenem-hydrolysing {beta}-lactamase KPC-2 in Klebsiella pneumoniae isolated in Rio de Janeiro, Brazil. J Antimicrob Chemother 63: 265-268 [Abstract] [Full Text]  
  • Navon-Venezia, S., Leavitt, A., Schwaber, M. J., Rasheed, J. K., Srinivasan, A., Patel, J. B., Carmeli, Y., and the Israeli KPC Kpn Study Group, (2009). First Report on a Hyperepidemic Clone of KPC-3-Producing Klebsiella pneumoniae in Israel Genetically Related to a Strain Causing Outbreaks in the United States. Antimicrob. Agents Chemother. 53: 818-820 [Abstract] [Full Text]  
  • Tenover, F. C., Emery, S. L., Spiegel, C. A., Bradford, P. A., Eells, S., Endimiani, A., Bonomo, R. A., McGowan, J. E. Jr. (2009). Identification of Plasmid-Mediated AmpC {beta}-Lactamases in Escherichia coli, Klebsiella spp., and Proteus Species Can Potentially Improve Reporting of Cephalosporin Susceptibility Testing Results. J. Clin. Microbiol. 47: 294-299 [Abstract] [Full Text]  
  • Cole, J. M., Schuetz, A. N., Hill, C. E., Nolte, F. S. (2009). Development and Evaluation of a Real-Time PCR Assay for Detection of Klebsiella pneumoniae Carbapenemase Genes. J. Clin. Microbiol. 47: 322-326 [Abstract] [Full Text]  
  • Marschall, J., Tibbetts, R. J., Dunne, W. M. Jr., Frye, J. G., Fraser, V. J., Warren, D. K. (2009). Presence of the KPC Carbapenemase Gene in Enterobacteriaceae Causing Bacteremia and Its Correlation with In Vitro Carbapenem Susceptibility. J. Clin. Microbiol. 47: 239-241 [Abstract] [Full Text]  
  • Woodford, N., Zhang, J., Warner, M., Kaufmann, M. E., Matos, J., MacDonald, A., Brudney, D., Sompolinsky, D., Navon-Venezia, S., Livermore, D. M. (2008). Arrival of Klebsiella pneumoniae producing KPC carbapenemase in the United Kingdom. J Antimicrob Chemother 62: 1261-1264 [Abstract] [Full Text]  
  • Doi, Y., Potoski, B. A., Adams-Haduch, J. M., Sidjabat, H. E., Pasculle, A. W., Paterson, D. L. (2008). Simple Disk-Based Method for Detection of Klebsiella pneumoniae Carbapenemase-Type {beta}-Lactamase by Use of a Boronic Acid Compound. J. Clin. Microbiol. 46: 4083-4086 [Abstract] [Full Text]  
  • Livermore, D. M., Hope, R., Brick, G., Lillie, M., Reynolds, R., on behalf of the BSAC Working Parties on Resistanc, (2008). Non-susceptibility trends among Enterobacteriaceae from bacteraemias in the UK and Ireland, 2001-06. J Antimicrob Chemother 62: ii41-ii54 [Abstract] [Full Text]  
  • Petrella, S., Ziental-Gelus, N., Mayer, C., Renard, M., Jarlier, V., Sougakoff, W. (2008). Genetic and Structural Insights into the Dissemination Potential of the Extremely Broad-Spectrum Class A {beta}-Lactamase KPC-2 Identified in an Escherichia coli Strain and an Enterobacter cloacae Strain Isolated from the Same Patient in France. Antimicrob. Agents Chemother. 52: 3725-3736 [Abstract] [Full Text]  
  • Hindiyeh, M., Smollen, G., Grossman, Z., Ram, D., Davidson, Y., Mileguir, F., Vax, M., Ben David, D., Tal, I., Rahav, G., Shamiss, A., Mendelson, E., Keller, N. (2008). Rapid Detection of blaKPC Carbapenemase Genes by Real-Time PCR. J. Clin. Microbiol. 46: 2879-2883 [Abstract] [Full Text]  
  • Cai, J. C., Zhou, H. W., Zhang, R., Chen, G.-X. (2008). Emergence of Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli Isolates Possessing the Plasmid-Mediated Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2 in Intensive Care Units of a Chinese Hospital. Antimicrob. Agents Chemother. 52: 2014-2018 [Abstract] [Full Text]  
  • Rasheed, J. K., Biddle, J. W., Anderson, K. F., Washer, L., Chenoweth, C., Perrin, J., Newton, D. W., Patel, J. B. (2008). Detection of the Klebsiella pneumoniae Carbapenemase Type 2 Carbapenem-Hydrolyzing Enzyme in Clinical Isolates of Citrobacter freundii and K. oxytoca Carrying a Common Plasmid. J. Clin. Microbiol. 46: 2066-2069 [Abstract] [Full Text]  
  • Naas, T., Cuzon, G., Villegas, M.-V., Lartigue, M.-F., Quinn, J. P., Nordmann, P. (2008). Genetic Structures at the Origin of Acquisition of the {beta}-Lactamase blaKPC Gene. Antimicrob. Agents Chemother. 52: 1257-1263 [Abstract] [Full Text]  
  • Schwaber, M. J., Klarfeld-Lidji, S., Navon-Venezia, S., Schwartz, D., Leavitt, A., Carmeli, Y. (2008). Predictors of Carbapenem-Resistant Klebsiella pneumoniae Acquisition among Hospitalized Adults and Effect of Acquisition on Mortality. Antimicrob. Agents Chemother. 52: 1028-1033 [Abstract] [Full Text]  
  • Zhang, R., Yang, L., Cai, J. C., Zhou, H. W., Chen, G.-X. (2008). High-level carbapenem resistance in a Citrobacter freundii clinical isolate is due to a combination of KPC-2 production and decreased porin expression. J Med Microbiol 57: 332-337 [Abstract] [Full Text]  
  • Dortet, L., Radu, I., Gautier, V., Blot, F., Chachaty, E., Arlet, G. (2008). Intercontinental travels of patients and dissemination of plasmid-mediated carbapenemase KPC-3 associated with OXA-9 and TEM-1. J Antimicrob Chemother 61: 455-457 [Full Text]  
  • Cuzon, G., Naas, T., Demachy, M. C., Nordmann, P. (2008). Plasmid-Mediated Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2 in Klebsiella pneumoniae Isolate from Greece. Antimicrob. Agents Chemother. 52: 796-797 [Full Text]  
  • Fonseca, F., Sarmento, A. C., Henriques, I., Samyn, B., van Beeumen, J., Domingues, P., Domingues, M. R., Saavedra, M. J., Correia, A. (2007). Biochemical Characterization of SFC-1, a Class A Carbapenem-Hydrolyzing {beta}-Lactamase. Antimicrob. Agents Chemother. 51: 4512-4514 [Abstract] [Full Text]  
  • Walther-Rasmussen, J., Hoiby, N. (2007). Class A carbapenemases. J Antimicrob Chemother 60: 470-482 [Abstract] [Full Text]  
  • Leavitt, A., Navon-Venezia, S., Chmelnitsky, I., Schwaber, M. J., Carmeli, Y. (2007). Emergence of KPC-2 and KPC-3 in Carbapenem-Resistant Klebsiella pneumoniae Strains in an Israeli Hospital. Antimicrob. Agents Chemother. 51: 3026-3029 [Abstract] [Full Text]  
  • Anderson, K. F., Lonsway, D. R., Rasheed, J. K., Biddle, J., Jensen, B., McDougal, L. K., Carey, R. B., Thompson, A., Stocker, S., Limbago, B., Patel, J. B. (2007). Evaluation of Methods To Identify the Klebsiella pneumoniae Carbapenemase in Enterobacteriaceae. J. Clin. Microbiol. 45: 2723-2725 [Abstract] [Full Text]  
  • Queenan, A. M., Bush, K. (2007). Carbapenemases: the Versatile {beta}-Lactamases. Clin. Microbiol. Rev. 20: 440-458 [Abstract] [Full Text]  
  • Villegas, M. V., Lolans, K., Correa, A., Kattan, J. N., Lopez, J. A., Quinn, J. P., and the Colombian Nosocomial Resistance Study Grou, (2007). First Identification of Pseudomonas aeruginosa Isolates Producing a KPC-Type Carbapenem-Hydrolyzing {beta}-Lactamase. Antimicrob. Agents Chemother. 51: 1553-1555 [Abstract] [Full Text]  
  • Kurupati, P., Tan, K. S. W., Kumarasinghe, G., Poh, C. L. (2007). Inhibition of Gene Expression and Growth by Antisense Peptide Nucleic Acids in a Multiresistant {beta}-Lactamase-Producing Klebsiella pneumoniae Strain. Antimicrob. Agents Chemother. 51: 805-811 [Abstract] [Full Text]  
  • Zhang, R., Zhou, H. W., Cai, J. C., Chen, G.-X. (2007). Plasmid-mediated carbapenem-hydrolysing {beta}-lactamase KPC-2 in carbapenem-resistant Serratia marcescens isolates from Hangzhou, China. J Antimicrob Chemother 59: 574-576 [Full Text]  
  • Wei, Z.-Q., Du, X.-X., Yu, Y.-S., Shen, P., Chen, Y.-G., Li, L.-J. (2007). Plasmid-Mediated KPC-2 in a Klebsiella pneumoniae Isolate from China. Antimicrob. Agents Chemother. 51: 763-765 [Abstract] [Full Text]  
  • Rodloff, A. C., Goldstein, E. J. C., Torres, A. (2006). Two decades of imipenem therapy. J Antimicrob Chemother 58: 916-929 [Abstract] [Full Text]  
  • Navon-Venezia, S., Chmelnitsky, I., Leavitt, A., Schwaber, M. J., Schwartz, D., Carmeli, Y. (2006). Plasmid-Mediated Imipenem-Hydrolyzing Enzyme KPC-2 among Multiple Carbapenem-Resistant Escherichia coli Clones in Israel.. Antimicrob. Agents Chemother. 50: 3098-3101 [Abstract] [Full Text]  
  • Virginia Villegas, M., Lolans, K., Correa, A., Jose Suarez, C., Lopez, J. A., Vallejo, M., Quinn, J. P., the Colombian Nosocomial Resistance Study Group, (2006). First Detection of the Plasmid-Mediated Class A Carbapenemase KPC-2 in Clinical Isolates of Klebsiella pneumoniae from South America.. Antimicrob. Agents Chemother. 50: 2880-2882 [Abstract] [Full Text]  
  • Mena, A., Plasencia, V., Garcia, L., Hidalgo, O., Ayestaran, J. I., Alberti, S., Borrell, N., Perez, J. L., Oliver, A. (2006). Characterization of a Large Outbreak by CTX-M-1-Producing Klebsiella pneumoniae and Mechanisms Leading to In Vivo Carbapenem Resistance Development.. J. Clin. Microbiol. 44: 2831-2837 [Abstract] [Full Text]  
  • Luzzaro, F., Mezzatesta, M., Mugnaioli, C., Perilli, M., Stefani, S., Amicosante, G., Rossolini, G. M., Toniolo, A. (2006). Trends in Production of Extended-Spectrum {beta}-Lactamases among Enterobacteria of Medical Interest: Report of the Second Italian Nationwide Survey.. J. Clin. Microbiol. 44: 1659-1664 [Abstract] [Full Text]  
  • Jacoby, G. A. (2006). {beta}-Lactamase Nomenclature.. Antimicrob. Agents Chemother. 50: 1123-1129 [Full Text]  
  • Lee, K., Yum, J. H., Yong, D., Lee, H. M., Kim, H. D., Docquier, J.-D., Rossolini, G. M., Chong, Y. (2005). Novel Acquired Metallo-{beta}-Lactamase Gene, blaSIM-1, in a Class 1 Integron from Acinetobacter baumannii Clinical Isolates from Korea. Antimicrob. Agents Chemother. 49: 4485-4491 [Abstract] [Full Text]  
  • Szabo, D., Melan, M. A., Hujer, A. M., Bonomo, R. A., Hujer, K. M., Bethel, C. R., Kristof, K., Paterson, D. L. (2005). Molecular Analysis of the Simultaneous Production of Two SHV-Type Extended-Spectrum Beta-Lactamases in a Clinical Isolate of Enterobacter cloacae by Using Single-Nucleotide Polymorphism Genotyping. Antimicrob. Agents Chemother. 49: 4716-4720 [Abstract] [Full Text]  
  • Alba, J., Ishii, Y., Thomson, K., Moland, E. S., Yamaguchi, K. (2005). Kinetics Study of KPC-3, a Plasmid-Encoded Class A Carbapenem-Hydrolyzing {beta}-Lactamase. Antimicrob. Agents Chemother. 49: 4760-4762 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Naas, T., Nordmann, P., Vedel, G., Poyart, C. (2005). Plasmid-Mediated Carbapenem-Hydrolyzing {beta}-Lactamase KPC in a Klebsiella pneumoniae Isolate from France. Antimicrob. Agents Chemother. 49: 4423-4424 [Full Text]  
  • Majiduddin, F. K., Palzkill, T. (2005). Amino Acid Residues That Contribute to Substrate Specificity of Class A {beta}-Lactamase SME-1. Antimicrob. Agents Chemother. 49: 3421-3427 [Abstract] [Full Text]  
  • Bratu, S., Mooty, M., Nichani, S., Landman, D., Gullans, C., Pettinato, B., Karumudi, U., Tolaney, P., Quale, J. (2005). Emergence of KPC-Possessing Klebsiella pneumoniae in Brooklyn, New York: Epidemiology and Recommendations for Detection. Antimicrob. Agents Chemother. 49: 3018-3020 [Abstract] [Full Text]  
  • Pitout, J. D. D., Gregson, D. B., Poirel, L., McClure, J.-A., Le, P., Church, D. L. (2005). Detection of Pseudomonas aeruginosa Producing Metallo-{beta}-Lactamases in a Large Centralized Laboratory. J. Clin. Microbiol. 43: 3129-3135 [Abstract] [Full Text]  
  • Bratu, S., Tolaney, P., Karumudi, U., Quale, J., Mooty, M., Nichani, S., Landman, D. (2005). Carbapenemase-producing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents. J Antimicrob Chemother 56: 128-132 [Abstract] [Full Text]  
  • Bratu, S., Landman, D., Haag, R., Recco, R., Eramo, A., Alam, M., Quale, J. (2005). Rapid Spread of Carbapenem-Resistant Klebsiella pneumoniae in New York City: A New Threat to Our Antibiotic Armamentarium. Arch Intern Med 165: 1430-1435 [Abstract] [Full Text]  
  • Walsh, T. R., Toleman, M. A., Poirel, L., Nordmann, P. (2005). Metallo-{beta}-Lactamases: the Quiet before the Storm?. Clin. Microbiol. Rev. 18: 306-325 [Abstract] [Full Text]  
  • Bratu, S., Landman, D., Alam, M., Tolentino, E., Quale, J. (2005). Detection of KPC Carbapenem-Hydrolyzing Enzymes in Enterobacter spp. from Brooklyn, New York. Antimicrob. Agents Chemother. 49: 776-778 [Abstract] [Full Text]  
  • Woodford, N., Tierno, P. M. Jr., Young, K., Tysall, L., Palepou, M.-F. I., Ward, E., Painter, R. E., Suber, D. F., Shungu, D., Silver, L. L., Inglima, K., Kornblum, J., Livermore, D. M. (2004). Outbreak of Klebsiella pneumoniae Producing a New Carbapenem- Hydrolyzing Class A {beta}-Lactamase, KPC-3, in a New York Medical Center. Antimicrob. Agents Chemother. 48: 4793-4799 [Abstract] [Full Text]  
  • Hossain, A., Ferraro, M. J., Pino, R. M., Dew, R. B. III, Moland, E. S., Lockhart, T. J., Thomson, K. S., Goering, R. V., Hanson, N. D. (2004). Plasmid-Mediated Carbapenem-Hydrolyzing Enzyme KPC-2 in an Enterobacter sp.. Antimicrob. Agents Chemother. 48: 4438-4440 [Abstract] [Full Text]  
  • Jacoby, G. A., Mills, D. M., Chow, N. (2004). Role of {beta}-Lactamases and Porins in Resistance to Ertapenem and Other {beta}-Lactams in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48: 3203-3206 [Abstract] [Full Text]  
  • Henriques, I., Moura, A., Alves, A., Saavedra, M. J., Correia, A. (2004). Molecular Characterization of a Carbapenem-Hydrolyzing Class A {beta}-Lactamase, SFC-1, from Serratia fonticola UTAD54. Antimicrob. Agents Chemother. 48: 2321-2324 [Abstract] [Full Text]  
  • Radice, M., Power, P., Gutkind, G., Fernandez, K., Vay, C., Famiglietti, A., Ricover, N., Ayala, J. A. (2004). First Class A Carbapenemase Isolated from Enterobacteriaceae in Argentina. Antimicrob. Agents Chemother. 48: 1068-1069 [Full Text]  
  • Poirel, L., Heritier, C., Tolun, V., Nordmann, P. (2004). Emergence of Oxacillinase-Mediated Resistance to Imipenem in Klebsiella pneumoniae. Antimicrob. Agents Chemother. 48: 15-22 [Abstract] [Full Text]  
  • Yigit, H., Queenan, A. M., Rasheed, J. K., Biddle, J. W., Domenech-Sanchez, A., Alberti, S., Bush, K., Tenover, F. C. (2003). Carbapenem-Resistant Strain of Klebsiella oxytoca Harboring Carbapenem-Hydrolyzing {beta}-Lactamase KPC-2. Antimicrob. Agents Chemother. 47: 3881-3889 [Abstract] [Full Text]  
  • Lee, K., Lim, Y. S., Yong, D., Yum, J. H., Chong, Y. (2003). Evaluation of the Hodge Test and the Imipenem-EDTA Double-Disk Synergy Test for Differentiating Metallo-{beta}-Lactamase-Producing Isolates of Pseudomonas spp. and Acinetobacter spp.. J. Clin. Microbiol. 41: 4623-4629 [Abstract] [Full Text]  
  • Miriagou, V., Tzouvelekis, L. S., Rossiter, S., Tzelepi, E., Angulo, F. J., Whichard, J. M. (2003). Imipenem Resistance in a Salmonella Clinical Strain Due to Plasmid-Mediated Class A Carbapenemase KPC-2. Antimicrob. Agents Chemother. 47: 1297-1300 [Abstract] [Full Text]  
  • Majiduddin, F. K., Palzkill, T. (2003). Amino Acid Sequence Requirements at Residues 69 and 238 for the SME-1 {beta}-Lactamase To Confer Resistance to {beta}-Lactam Antibiotics. Antimicrob. Agents Chemother. 47: 1062-1067 [Abstract] [Full Text]  
  • Smith Moland, E., Hanson, N. D., Herrera, V. L., Black, J. A., Lockhart, T. J., Hossain, A., Johnson, J. A., Goering, R. V., Thomson, K. S. (2003). Plasmid-mediated, carbapenem-hydrolysing {beta}-lactamase, KPC-2, in Klebsiella pneumoniae isolates. J Antimicrob Chemother 51: 711-714 [Abstract] [Full Text]  
  • Poirel, L., Heritier, C., Podglajen, I., Sougakoff, W., Gutmann, L., Nordmann, P. (2003). Emergence in Klebsiella pneumoniae of a Chromosome-Encoded SHV {beta}-Lactamase That Compromises the Efficacy of Imipenem. Antimicrob. Agents Chemother. 47: 755-758 [Abstract] [Full Text]  
  • Steward, C. D., Mohammed, J. M., Swenson, J. M., Stocker, S. A., Williams, P. P., Gaynes, R. P., McGowan, J. E. Jr., Tenover, F. C. (2003). Antimicrobial Susceptibility Testing of Carbapenems: Multicenter Validity Testing and Accuracy Levels of Five Antimicrobial Test Methods for Detecting Resistance in Enterobacteriaceae and Pseudomonas aeruginosa Isolates. J. Clin. Microbiol. 41: 351-358 [Abstract] [Full Text]  
  • Poirel, L., Weldhagen, G. F., Naas, T., De Champs, C., Dove, M. G., Nordmann, P. (2001). GES-2, a Class A {beta}-Lactamase from Pseudomonas aeruginosa with Increased Hydrolysis of Imipenem. Antimicrob. Agents Chemother. 45: 2598-2603 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»