This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Riccio, M. L.
Right arrow Articles by Rossolini, G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Riccio, M. L.
Right arrow Articles by Rossolini, G. M.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, April 2001, p. 1249-1253, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1249-1253.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

In70 of Plasmid pAX22, a blaVIM-1-Containing Integron Carrying a New Aminoglycoside Phosphotransferase Gene Cassette

Maria Letizia Riccio,1 Lucia Pallecchi,1 Roberta Fontana,2 and Gian Maria Rossolini1,*

Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100 Siena,1 and Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona, I-37134 Verona,2 Italy

Received 7 August 2000/Returned for modification 18 December 2000/Accepted 26 January 2001

An Achromobacter xylosoxydans strain showing broad-spectrum resistance to beta -lactams (including carbapenems) and aminoglycosides was isolated at the University Hospital of Verona (Verona, Italy). This strain was found to produce metallo-beta -lactamase activity and to harbor a 30-kb nonconjugative plasmid, named pAX22, carrying a blaVIM-1 determinant inserted into a class 1 integron. Characterization of this integron, named In70, revealed an original array of four gene cassettes containing, respectively, the blaVIM-1 gene and three different aminoglycoside resistance determinants, including an aacA4 allele, a new aph-like gene named aphA15, and an aadA1 allele. The aphA15 gene is the first example of an aph-like gene carried on a mobile gene cassette, and its product exhibits close similarity to the APH(3')-IIa aminoglycoside phosphotransferase encoded by Tn5 (36% amino acid identity) and to an APH(3')-IIb enzyme from Pseudomonas aeruginosa (38% amino acid identity). Expression of the cloned aphA15 gene in Escherichia coli reduced the susceptibility to kanamycin and neomycin as well as (slightly) to amikacin, netilmicin, and streptomycin. Characterization of the 5' and 3' conserved segments of In70 and of their flanking regions showed that In70 belongs to the group of class 1 integrons associated with defective transposon derivatives originating from Tn402-like elements. The structure of the 3' conserved segment indicates the closest ancestry with members of the In0-In2 lineage. In70, with its array of cassette-borne resistance genes, can mediate broad-spectrum resistance to most beta -lactams and aminoglycosides.

* Corresponding author. Mailing address: Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, Via Laterina, 8, 53100 Siena, Italy. Phone: 39 0577 233327. Fax: 39 0577 233325. E-mail: rossolini{at}unisi.it.

Antimicrobial Agents and Chemotherapy, April 2001, p. 1249-1253, Vol. 45, No. 4
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.4.1249-1253.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Strateva, T., Yordanov, D. (2009). Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 58: 1133-1148 [Abstract] [Full Text]  
  • Dam, B., Ghosh, W., Das Gupta, S. K. (2009). Conjugative Type 4 Secretion System of a Novel Large Plasmid from the Chemoautotroph Tetrathiobacter kashmirensis and Construction of Shuttle Vectors for Alcaligenaceae. Appl. Environ. Microbiol. 75: 4362-4373 [Abstract] [Full Text]  
  • Daikos, G. L., Petrikkos, P., Psichogiou, M., Kosmidis, C., Vryonis, E., Skoutelis, A., Georgousi, K., Tzouvelekis, L. S., Tassios, P. T., Bamia, C., Petrikkos, G. (2009). Prospective Observational Study of the Impact of VIM-1 Metallo-{beta}-Lactamase on the Outcome of Patients with Klebsiella pneumoniae Bloodstream Infections. Antimicrob. Agents Chemother. 53: 1868-1873 [Abstract] [Full Text]  
  • Siarkou, V. I., Vitti, D., Protonotariou, E., Ikonomidis, A., Sofianou, D. (2009). Molecular Epidemiology of Outbreak-Related Pseudomonas aeruginosa Strains Carrying the Novel Variant blaVIM-17 Metallo-{beta}-Lactamase Gene. Antimicrob. Agents Chemother. 53: 1325-1330 [Abstract] [Full Text]  
  • Papagiannitsis, C. C., Tzouvelekis, L. S., Miriagou, V. (2009). Relative Strengths of the Class 1 Integron Promoter Hybrid 2 and the Combinations of Strong and Hybrid 1 with an Active P2 Promoter. Antimicrob. Agents Chemother. 53: 277-280 [Abstract] [Full Text]  
  • Rossolini, G. M., Luzzaro, F., Migliavacca, R., Mugnaioli, C., Pini, B., De Luca, F., Perilli, M., Pollini, S., Spalla, M., Amicosante, G., Toniolo, A., Pagani, L. (2008). First Countrywide Survey of Acquired Metallo-{beta}-Lactamases in Gram-Negative Pathogens in Italy. Antimicrob. Agents Chemother. 52: 4023-4029 [Abstract] [Full Text]  
  • Juan, C., Beceiro, A., Gutierrez, O., Alberti, S., Garau, M., Perez, J. L., Bou, G., Oliver, A. (2008). Characterization of the New Metallo-{beta}-Lactamase VIM-13 and Its Integron-Borne Gene from a Pseudomonas aeruginosa Clinical Isolate in Spain. Antimicrob. Agents Chemother. 52: 3589-3596 [Abstract] [Full Text]  
  • Li, H., Toleman, M. A., Bennett, P. M., Jones, R. N., Walsh, T. R. (2008). Complete Sequence of p07-406, a 24,179-Base-Pair Plasmid Harboring the blaVIM-7 Metallo-{beta}-Lactamase Gene in a Pseudomonas aeruginosa Isolate from the United States. Antimicrob. Agents Chemother. 52: 3099-3105 [Abstract] [Full Text]  
  • Doi, Y., Poirel, L., Paterson, D. L., Nordmann, P. (2008). Characterization of a Naturally Occurring Class D {beta}-Lactamase from Achromobacter xylosoxidans. Antimicrob. Agents Chemother. 52: 1952-1956 [Abstract] [Full Text]  
  • Aschbacher, R., Doumith, M., Livermore, D. M., Larcher, C., Woodford, N. (2008). Linkage of acquired quinolone resistance (qnrS1) and metallo-{beta}-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy. J Antimicrob Chemother 61: 515-523 [Abstract] [Full Text]  
  • Gutierrez, O., Juan, C., Cercenado, E., Navarro, F., Bouza, E., Coll, P., Perez, J. L., Oliver, A. (2007). Molecular Epidemiology and Mechanisms of Carbapenem Resistance in Pseudomonas aeruginosa Isolates from Spanish Hospitals. Antimicrob. Agents Chemother. 51: 4329-4335 [Abstract] [Full Text]  
  • Libisch, B., Muzslay, M., Gacs, M., Minarovits, J., Knausz, M., Watine, J., Ternak, G., Kenez, E., Kustos, I., Rokusz, L., Szeles, K., Balogh, B., Fuzi, M. (2006). Molecular Epidemiology of VIM-4 Metallo-{beta}-Lactamase-Producing Pseudomonas sp. Isolates in Hungary. Antimicrob. Agents Chemother. 50: 4220-4223 [Abstract] [Full Text]  
  • Yu, Y.-S., Qu, T.-T., Zhou, J.-Y., Wang, J., Li, H.-Y., Walsh, T. R. (2006). Integrons Containing the VIM-2 Metallo-{beta}-Lactamase Gene among Imipenem-Resistant Pseudomonas aeruginosa Strains from Different Chinese Hospitals. J. Clin. Microbiol. 44: 4242-4245 [Abstract] [Full Text]  
  • Lagatolla, C., Edalucci, E., Dolzani, L., Riccio, M. L., De Luca, F., Medessi, E., Rossolini, G. M., Tonin, E. A. (2006). Molecular Evolution of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa in a Nosocomial Setting of High-Level Endemicity.. J. Clin. Microbiol. 44: 2348-2353 [Abstract] [Full Text]  
  • Pournaras, S., Ikonomidis, A., Tzouvelekis, L. S., Tokatlidou, D., Spanakis, N., Maniatis, A. N., Legakis, N. J., Tsakris, A. (2005). VIM-12, a Novel Plasmid-Mediated Metallo-{beta}-Lactamase from Klebsiella pneumoniae That Resembles a VIM-1/VIM-2 Hybrid. Antimicrob. Agents Chemother. 49: 5153-5156 [Abstract] [Full Text]  
  • Mantengoli, E., Rossolini, G. M. (2005). Tn5393d, a Complex Tn5393 Derivative Carrying the PER-1 Extended-Spectrum {beta}-Lactamase Gene and Other Resistance Determinants. Antimicrob. Agents Chemother. 49: 3289-3296 [Abstract] [Full Text]  
  • Giuliani, F., Docquier, J.-D., Riccio, M. L., Pagani, L., Rossolini, G. M. (2005). OXA-46, a New Class D {beta}-Lactamase of Narrow Substrate Specificity Encoded by a blaVIM-1-Containing Integron from a Pseudomonas aeruginosa Clinical Isolate. Antimicrob. Agents Chemother. 49: 1973-1980 [Abstract] [Full Text]  
  • Walsh, T. R., Toleman, M. A., Poirel, L., Nordmann, P. (2005). Metallo-{beta}-Lactamases: the Quiet before the Storm?. Clin. Microbiol. Rev. 18: 306-325 [Abstract] [Full Text]  
  • Riccio, M. L., Pallecchi, L., Docquier, J.-D., Cresti, S., Catania, M. R., Pagani, L., Lagatolla, C., Cornaglia, G., Fontana, R., Rossolini, G. M. (2005). Clonal Relatedness and Conserved Integron Structures in Epidemiologically Unrelated Pseudomonas aeruginosa Strains Producing the VIM-1 Metallo-{beta}-Lactamase from Different Italian Hospitals. Antimicrob. Agents Chemother. 49: 104-110 [Abstract] [Full Text]  
  • Aubert, D., Girlich, D., Naas, T., Nagarajan, S., Nordmann, P. (2004). Functional and Structural Characterization of the Genetic Environment of an Extended-Spectrum {beta}-Lactamase blaVEB Gene from a Pseudomonas aeruginosa Isolate Obtained in India. Antimicrob. Agents Chemother. 48: 3284-3290 [Abstract] [Full Text]  
  • Libisch, B., Gacs, M., Csiszar, K., Muzslay, M., Rokusz, L., Fuzi, M. (2004). Isolation of an Integron-Borne blaVIM-4 Type Metallo-{beta}-Lactamase Gene from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate in Hungary. Antimicrob. Agents Chemother. 48: 3576-3578 [Abstract] [Full Text]  
  • Vakulenko, S. B., Mobashery, S. (2003). Versatility of Aminoglycosides and Prospects for Their Future. Clin. Microbiol. Rev. 16: 430-450 [Abstract] [Full Text]  
  • Miriagou, V., Tzelepi, E., Gianneli, D., Tzouvelekis, L. S. (2003). Escherichia coli with a Self-Transferable, Multiresistant Plasmid Coding for Metallo-{beta}-Lactamase VIM-1. Antimicrob. Agents Chemother. 47: 395-397 [Abstract] [Full Text]  
  • Pournaras, S., Tsakris, A., Maniati, M., Tzouvelekis, L. S., Maniatis, A. N. (2002). Novel Variant (blaVIM-4) of the Metallo-{beta}-Lactamase Gene blaVIM-1 in a Clinical Strain of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 46: 4026-4028 [Abstract] [Full Text]  
  • Lombardi, G., Luzzaro, F., Docquier, J.-D., Riccio, M. L., Perilli, M., Coli, A., Amicosante, G., Rossolini, G. M., Toniolo, A. (2002). Nosocomial Infections Caused by Multidrug-Resistant Isolates of Pseudomonas putida Producing VIM-1 Metallo-{beta}-Lactamase. J. Clin. Microbiol. 40: 4051-4055 [Abstract] [Full Text]  
  • Partridge, S. R., Collis, C. M., Hall, R. M. (2002). Class 1 Integron Containing a New Gene Cassette, aadA10, Associated with Tn1404 from R151. Antimicrob. Agents Chemother. 46: 2400-2408 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»