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Antimicrobial Agents and Chemotherapy, May 2001, p. 1550-1552, Vol. 45, No. 5
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.5.1550-1552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Absence of Mutations in marRAB or soxRS in acrB-Overexpressing Fluoroquinolone-Resistant Clinical and Veterinary Isolates of Escherichia coli

Mark A. Webber and Laura J. V. Piddock*

Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom

Received 7 September 2000/Returned for modification 2 November 2000/Accepted 30 January 2001

The amount of acrB, marA, and soxS mRNA was determined in 36 fluoroquinolone-resistant E. coli from humans and animals, 27 of which displayed a multiple-resistance phenotype. acrB mRNA was elevated in 11 of 36 strains. A mutation at codon 45 (Argright-arrowCys) in acrR was found in 6 of these 11 strains. Ten of the 36 isolates appeared to overexpress soxS, and five appeared to overexpress marA. A number of mutations were found in the marR and soxR repressor genes, correlating with greater amounts of marA and soxS mRNA, respectively.

* Corresponding author. Mailing address: Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, Birmingham B15 2TT, United Kingdom. Phone: 0121-414-6969. Fax: 0121-414-6966. E-mail: l.j.v.piddock{at}bham.ac.uk.

Antimicrobial Agents and Chemotherapy, May 2001, p. 1550-1552, Vol. 45, No. 5
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.5.1550-1552.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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