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Antimicrobial Agents and Chemotherapy, June 2001, p. 1700-1704, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1700-1704.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Proinflammatory Activity of a Cecropin-Like Antibacterial Peptide from Helicobacter pylori

Johan Bylund,1,* Thierry Christophe,2 Francois Boulay,2 Thomas Nyström,3 Anna Karlsson,1 and Claes Dahlgren1

The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology,1 and Department of Cell and Molecular Biology-Microbiology,3 University of Göteborg, Göteborg, Sweden, and DBMS/BBSI (UMR 5092 CEA/CNRS/UJF) CEA-Grenoble, France2

Received 23 October 2000/Returned for modification 23 January 2001/Accepted 1 March 2001

Helicobacter pylori, the bacterial pathogen associated with gastritis and peptic ulcers, is highly successful in establishing infection in the human gastric mucosa, a process typically associated with massive infiltration of inflammatory cells. Colonization of the mucosa is suggested to be facilitated by H. pylori-produced cecropin-like peptides with antibacterial properties, giving the microbe a competitive advantage over other bacteria. We show that a cecropin-like antibacterial peptide from H. pylori, Hp(2-20), not only has a potent bactericidal effect but also induces proinflammatory activities in human neutrophils, e.g., upregulation of integrins (Mac-1), induction of chemotaxis, and activation of the oxygen radical producing NADPH-oxidase. Furthermore, we show that these effects are mediated through binding of Hp(2-20) to the promiscuous, G-protein-linked lipoxin A4 receptor-formyl peptide-like receptor 1.

* Corresponding author. Mailing address: The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Göteborg, Box 435, Göteborg, S-405 30 Sweden. Phone: 46-313424471. Fax: 46-31828898. E-mail: Johan.Bylund{at}microbio.gu.se.

Antimicrobial Agents and Chemotherapy, June 2001, p. 1700-1704, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1700-1704.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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