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Antimicrobial Agents and Chemotherapy, June 2001, p. 1900-1904, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1900-1904.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Staphylococcus aureus Cell Extract Transcription-Translation Assay: Firefly Luciferase Reporter System for Evaluating Protein Translation Inhibitors

Robert W. Murray, Earline P. Melchior, Jeanne C. Hagadorn, and Keith R. Marotti*

Pharmacia Corporation, Kalamazoo, Michigan 49007

Received 1 December 2000/Returned for modification 25 January 2001/Accepted 9 March 2001

The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extract S. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.

* Corresponding author. Mailing address: Infectious Diseases Biology, 7263-209-721, 301 Henrietta St., Kalamazoo, MI 49007. Phone: (616) 833-1934. Fax: (616) 833-1559. E-mail: Keith.R.Marotti{at}pharmacia.com.

Antimicrobial Agents and Chemotherapy, June 2001, p. 1900-1904, Vol. 45, No. 6
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.6.1900-1904.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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