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Antimicrobial Agents and Chemotherapy, July 2001, p. 2154-2156, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2154-2156.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Resistance to Linezolid: Characterization of Mutations in rRNA and Comparison of Their Occurrences in Vancomycin-Resistant Enterococci

Jason Prystowsky,1 Farida Siddiqui,1 John Chosay,2 Dean L. Shinabarger,2 John Millichap,3 Lance R. Peterson,3,4 and Gary A. Noskin1,4,*

Department of Medicine, Division of Infectious Diseases,1 and Department of Pathology, Clinical Microbiology Division,3 Northwestern University Medical School, and the Prevention Epicenter, Northwestern Memorial Hospital,4 Chicago, Illinois, and Pharmacia Corporation, Kalamazoo, Michigan2

Received 11 December 2000/Returned for modification 29 January 2001/Accepted 28 March 2001

To assess the potential for emergence of resistance during the use of linezolid, we tested 10 clinical isolates of vancomycin-resistant enterococci (VRE) (four Enterococcus faecalis, five Enterococcus faecium, and one Enterococcus gallinarum) as well as a vancomycin-susceptible control (ATCC 29212) strain of E. faecalis. The enterococci were exposed to doubling dilutions of linezolid for 12 passes. After the final passage, the linezolid plate growing VRE contained a higher drug concentration with E. faecalis than with E. faecium. DNA sequencing of the 23S rRNA genes revealed that linezolid resistance in three E. faecalis isolates was associated with a guanine to uracil transversion at bp 2576, while the one E. faecium isolate for which the MIC was 16 µg/ml contained a guanine to adenine transition at bp 2505.

* Corresponding author. Mailing address: Division of Infectious Diseases, Northwestern University Medical School, Feinberg 16-704, 251 E. Huron St., Chicago, IL 60611. Phone: (312) 926-2729. Fax: (312) 926-7845. E-mail: gnoskin{at}northwestern.edu.

Antimicrobial Agents and Chemotherapy, July 2001, p. 2154-2156, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2154-2156.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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