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Antimicrobial Agents and Chemotherapy, July 2001, p. 2157-2159, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2157-2159.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Mutations in ndh in Isoniazid-Resistant Mycobacterium tuberculosis Isolates

Ann S. G. Lee,1,2,* Audrey S. M. Teo,3 and Sin-Yew Wong3

Department of Clinical Research, Singapore General Hospital, Singapore 169608,1 Division of Medical Sciences, National Cancer Centre, Singapore 169610,2 and Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore 308433,3 Singapore

Received 11 December 2000/Returned for modification 13 February 2001/Accepted 26 April 2001

Novel mutations in NADH dehydrogenase (ndh) were detected in 8 of 84 (9.5%) isoniazid (INH)-resistant isolates (T110A [n = 1], R268H [n = 7]), but not in 22 INH-susceptible isolates of Mycobacterium tuberculosis. Significantly, all eight isolates with mutations at ndh did not have mutations at katG, kasA, or the promoter regions of inhA or ahpC, except for one isolate. Mutations in ndh appear to be an additional molecular mechanism for isoniazid resistance in M. tuberculosis.

* Corresponding author. Mailing address: Division of Medical Sciences, National Cancer Centre, Republic of Singapore 169610. Phone: 65-436 8313. Fax: 65-372 0161. E-mail: dmslsg{at}nccs.com.sg.

Antimicrobial Agents and Chemotherapy, July 2001, p. 2157-2159, Vol. 45, No. 7
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.7.2157-2159.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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