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Antimicrobial Agents and Chemotherapy, August 2001, p. 2198-2203, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2198-2203.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Growth and Development of Tetracycline-Resistant Chlamydia suis†

J. Lenart,1 A. A. Andersen,2 and D. D. Rockey1,*

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331,1 and National Animal Disease Center, USDA Agriculture Research Service, Ames, Iowa 500102

Received 20 November 2000/Returned for modification 26 February 2001/Accepted 5 May 2001

Tetracycline (TET) is a front-line antibiotic for the treatment of chlamydial infections in both humans and animals, and the emergence of TET-resistant (Tetr) Chlamydia is of significant clinical importance. Recently, several Tetr chlamydial strains have been isolated from swine (Sus scrofa) raised in production facilities in Nebraska. Here, the intracellular development of two Tetr strains, R19 and R27, is characterized through the use of tissue culture and immunofluorescence. The strains grow in concentrations of up to 4 µg of TET/ml, while a TET-sensitive (Tets) swine strain (S45) and a strain of the human serovar L2 (LGV-434) grow in up to 0.1 µg of TET/ml. Although inclusions form in the presence of TET, many contain large aberrant reticulate bodies (RBs) that do not differentiate into infectious elementary bodies. The percentage of inclusions containing typical developmental forms decreases with increasing TET concentrations, and at 3 µg of TET/ml 100% of inclusions contain aberrant RBs. However, upon removal of TET the aberrant RBs revert to typical RBs, and a productive developmental cycle ensues. In addition, inclusions were found that contained both C. suis R19 and Chlamydia trachomatis L2 after sequential infection, demonstrating that two biologically distinct chlamydial strains could both develop within a single inclusion.

* Corresponding author. Mailing address: Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331. Phone: (541) 737-1848. Fax: (541) 737-0496. E-mail: rockeyd{at}ucs.orst.edu.

dagger Oregon Agricultural Experimental Station Technical Paper 11775.

Antimicrobial Agents and Chemotherapy, August 2001, p. 2198-2203, Vol. 45, No. 8
0066-4804/01/$04.00+0   DOI: 10.1128/AAC.45.8.2198-2203.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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