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Antimicrobial Agents and Chemotherapy, January 2002, p. 34-41, Vol. 46, No. 1
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.1.34-41.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Vitro Microbicidal Activities of Cecropin Peptides D2A21 and D4E1 and Gel Formulations Containing 0.1 to 2% D2A21 against Chlamydia trachomatis

L. M. Ballweber,1 J. E. Jaynes,2 W. E. Stamm,3 and M. F. Lampe3,4*

Departments of Obstetrics and Gynecology,1 Demegen, Inc., Pittsburgh, Pennsylvania,2 Division of Allergy and Infectious Diseases, Department of Medicine, University of Washington, Seattle, Washington,3 Laboratory Medicine4

Received 1 March 2001/ Returned for modification 18 April 2001/ Accepted 20 September 2001

Topically applied microbicides that eradicate pathogens at the time of initial exposure represent a powerful strategy for the prevention of sexually transmitted infections. To aid in the further development of an effective topical microbicide, we assessed the minimum cidal concentration (MCC) of two cecropin peptides, D2A21 and D4E1, and gel formulations containing 0.1 to 2% D2A21 against Chlamydia trachomatis in vitro. The MCC of peptide D2A21was 5 µM (18.32 µg/ml), and that of peptide D4E1 was 7.5 µM (21.69 µg/ml). The MCC of gel formulations containing 2% D2A21 was 0.2 mM (0.7 mg/ml), and that of gel formulations containing 0.5% D2A21 was 0.2 mM (0.7 mg/ml). There was no significant variation in the results when two different C. trachomatis strains were tested, and the addition of 10% human blood did not significantly alter the MCCs. pH values above and below 7 reduced the activity of the D2A21 peptide alone, but the MCC of the 2% D2A21 gel formulation was only slightly altered at the various pHs tested. Ultrastructural studies indicated that C. trachomatis membranes were disrupted after D2A21 exposure, resulting in leakage of the cytoplasmic contents. These in vitro results suggest that these cecropin peptides may be an effective topical microbicide against C. trachomatis and support the need for further evaluation.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Box 357110, University of Washington, Seattle, WA 98195. Phone: (206) 598-2135. Fax: (206) 598-6189. E-mail: lampe{at}u.washington.edu.

Antimicrobial Agents and Chemotherapy, January 2002, p. 34-41, Vol. 46, No. 1
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.1.34-41.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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