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Antimicrobial Agents and Chemotherapy, October 2002, p. 3265-3267, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3265-3267.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

-11 Mutation in the ampC Promoter Increasing Resistance to ß-Lactams in a Clinical Escherichia coli Strain

S. Corvec,1* N. Caroff,2 E. Espaze,1 J. Marraillac,1 and A. Reynaud2

Laboratoire de Bactériologie-Virologie, Hygiène hospitalière, CHU,1 Laboratoire de Bactériologie-Virologie, Faculté de Pharmacie, Université de Nantes, Nantes, France2

Received 25 January 2002/ Returned for modification 6 May 2002/ Accepted 19 July 2002

A mutation was discovered in the Pribnow box of the ampC promoter in a clinical Escherichia coli strain. This -11 C-to-T transition created a perfect homology with the -10 consensus sequence. The new promoter was cloned upstream of the cat gene of pKK232-8 and induced a sixfold increase in promoter strength.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie-Hygiène hospitalière, CHU de Nantes, 9 quai Moncousu, 44093 Nantes Cedex 01, France. Phone: 33.2.40.08.39.86. Fax: 33.2.40.08.38.29. E-mail: stephane.corvec{at}chu-nantes.fr.

Antimicrobial Agents and Chemotherapy, October 2002, p. 3265-3267, Vol. 46, No. 10
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.10.3265-3267.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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