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Antimicrobial Agents and Chemotherapy, November 2002, p. 3657-3659, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3657-3659.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations in Ribosomal Protein L16 and in 23S rRNA in Enterococcus Strains for Which Evernimicin MICs Differ

Myriam Zarazaga, Carmen Tenorio, Rosa Del Campo, Fernanda Ruiz-Larrea, and Carmen Torres*

Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain

Received 2 April 2002/ Returned for modification 23 May 2002/ Accepted 26 July 2002

Mutations in ribosomal protein L16 and in 23S rRNA were investigated in 22 Enterococcus strains of different species and for which the MICs of evernimicin differ (MICs, 0.023 to 16 µg/ml). Amino acid changes (Arg56His, Ile52Thr, or Arg51His) in protein L16 were found in seven strains, and a nucleotide G2535A mutation in 23S rRNA was found in 1 strain among 13 for which the MICs are >=1 µg/ml.

* Corresponding author. Mailing address: Area de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios, 51, 26006 Logroño, Spain. Phone: 34-941299750. Fax: 34-941299721. E-mail: carmen.torres{at}daa.unirioja.es.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3657-3659, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3657-3659.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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