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Antimicrobial Agents and Chemotherapy, November 2002, p. 3660-3664, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3660-3664.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Class 1 Integron-Associated Tobramycin-Gentamicin Resistance in Campylobacter jejuni Isolated from the Broiler Chicken House Environment

Margie D. Lee,1* Susan Sanchez,2 Martha Zimmer,3 Umelaalim Idris,1 Mark E. Berrang,4 and Patrick F. McDermott5

Department of Medical Microbiology and Parasitology,1 Athens Diagnostic Laboratory,2 Department of Environmental Health Sciences, The University of Georgia, Athens, Georgia 30602,3 Poultry Production and Meat Quality, U.S. Department of Agriculture, Athens, Georgia 30604,4 Center for Veterinary Medicine, Food and Drug Administration, Laurel, Maryland 207085

Received 18 December 2001/ Returned for modification 15 April 2002/ Accepted 27 July 2002

Using PCR, we screened 105 isolates of poultry-associated Campylobacter jejuni for the presence of class 1 integrons. Of those isolates, 21% (22 of 105) possessed the integrase gene, but only 5 isolates produced an amplicon in a 5'-3' conserved sequence PCR directed toward amplification of the resistance cassettes. DNA sequencing demonstrated that all five isolates possessed the aminoglycoside resistance gene, aacA4.

* Corresponding author. Mailing address: Department of Medical Microbiology and Parasitology, The University of Georgia, Athens, GA 30602. Phone: (706) 542-5778. Fax: (706) 542-5771. E-mail: leem{at}vet.uga.edu.

Antimicrobial Agents and Chemotherapy, November 2002, p. 3660-3664, Vol. 46, No. 11
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.11.3660-3664.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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