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Antimicrobial Agents and Chemotherapy, December 2002, p. 3739-3743, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3739-3743.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Distribution of Extended-Spectrum ß-Lactamases in Clinical Isolates of Enterobacteriaceae in Vietnam

Van Cao,1,2 Thierry Lambert,1,3* Duong Quynh Nhu,2 Huynh Kim Loan,2 Nguyen Kim Hoang,2 Guillaume Arlet,4 and Patrice Courvalin1

Unité des Agents Antibactériens, Institut Pasteur, 75724 Paris Cedex 15,1 Centre d'Etude Pharmaceutiques, ChÂtenay-Malabry,3 Service de Bactériologie, Hôpital Tenon, U.F.R Saint-Antoine 75970 Paris Cedex 20, France,4 Institut Pasteur d'Ho Chi Minh Ville, Ho Chi Minh City, Vietnam2

Received 4 February 2002/ Returned for modification 20 May 2002/ Accepted 21 August 2002

Among 730 Escherichia coli, 438 Klebsiella pneumoniae, and 141 Proteus mirabilis isolates obtained between September 2000 and September 2001 in seven hospitals in Ho Chi Minh City, Vietnam, 26.6% were resistant to ceftazidime, 30% were resistant to cefotaxime, 31.5% were resistant to ceftriaxone, 15.9% were resistant to cefoperazone, and 6% were resistant to cefepime. Resistance to imipenem was found in 5.6% of the isolates. In 55 strains producing extended-spectrum ß-lactamases (32 E. coli isolates, 13 K. pneumoniae isolates, and 10 P. mirabilis isolates), structural genes for VEB-1 (25.5%), CTX-M (25.5%), SHV (38.1%), and TEM (76.3%) enzymes were detected alone or in combination. Sequencing of the PCR products obtained from the K. pneumoniae isolates revealed the presence of blaVEB-1, blaCTX-M-14, blaCTX-M-17, blaSHV-2, and blaTEM-1. Molecular typing of the strains with a similar resistance phenotype to broad-spectrum cephalosporins indicated polyclonal spread. ISEcp1 was presumably responsible for dissemination of the blaCTX-M-like gene.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 25, rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 45 68 83 20. Fax: (33) 1 45 68 83 19. E-mail address: tlambert{at}pasteur.fr.

Antimicrobial Agents and Chemotherapy, December 2002, p. 3739-3743, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3739-3743.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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