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Antimicrobial Agents and Chemotherapy, December 2002, p. 3744-3749, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3744-3749.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

Satoshi Ameyama,1* Shoichi Onodera,2 Masahiro Takahata,1 Shinzaburo Minami,1 Nobuko Maki,3 Katsuhisa Endo,4 Hirokazu Goto,2 Hiroo Suzuki,5 and Yukihiko Oishi2

Research Laboratories, Toyama Chemical Co., Ltd., 2-4-1, Shimookui, Toyama, 930-8508,1 Department of Urology, Jikei University, School of Medicine, 3-19-18, Nishishinbashi, Minato-ku, Tokyo, 105-8741,2 Medical Information Center, Toyama Chemical Co., Ltd., 3-2-5, Nishishinjuku, Shinjuku-ku, Tokyo 160-0023,3 Department of Urology, JR Tokyo General Hospital, 2-1-3, Yoyogi, Shibuya-ku, Tokyo, 151-8528,4 Department of Urology, Kosei Hospital, 5-25-15, Yayoi-cho, Nakano-ku, Tokyo, 164-8617, Japan5

Received 30 April 2002/ Returned for modification 9 July 2002/ Accepted 22 August 2002

Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 µg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 µg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

* Corresponding author. Mailing address: Research Laboratories, Toyama Chemical Co., Ltd., 2-4-1, Shimookui, Toyama, 930-8508, Japan. Phone: 81-764-31-8268. Fax: 81-764-31-8208. E-mail: SATOSHI_AMEYAMA{at}toyama-chemical.co.jp.

Antimicrobial Agents and Chemotherapy, December 2002, p. 3744-3749, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3744-3749.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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