This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moland, E. S.
Right arrow Articles by Thomson, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moland, E. S.
Right arrow Articles by Thomson, K. S.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, December 2002, p. 3837-3842, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3837-3842.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Occurrence of Newer ß-Lactamases in Klebsiella pneumoniae Isolates from 24 U.S. Hospitals

Ellen Smith Moland, Jennifer A. Black, Jason Ourada, Mark D. Reisbig, Nancy D. Hanson, and Kenneth S. Thomson*

Center for Research in Antiinfectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178

Received 22 April 2002/ Returned for modification 17 May 2002/ Accepted 29 August 2002

Despite the discovery of novel ß-lactamases such as extended-spectrum ß-lactamases (ESBLs), imported AmpC, and carbapenem-hydrolyzing ß-lactamases at least a decade ago, there remains a low level of awareness of their importance and how to detect them. There is a need to increase the levels of awareness of clinical laboratories about the detection of newer ß-lactamases. Therefore, a study was conducted in 2000 to investigate the occurrence of these ß-lactamases in Klebsiella pneumoniae isolates at 24 U.S. medical centers. To enhance the likelihood of detecting imported AmpC and carbapenem-hydrolyzing ß-lactamases, participating laboratories were permitted to include archived strains (1996 to 2000) that were intermediate or resistant to either cefoxitin or imipenem. The ß-lactamase production of 408 isolates positive by screening of 1,123 isolates was investigated by ESBL phenotypic confirmation tests; and for AmpC and carbapenem-hydrolyzing ß-lactamases, three-dimensional tests, isoelectric focusing, ß-lactamase inhibitor studies, spectrophotometric assays, induction assays, and molecular tests were used. ESBL-producing isolates were detected at 18 of the 24 sites (75%), imported AmpC-producing isolates were detected at 10 sites (42%), inducible imported AmpC-producing isolates were detected at 3 sites (12.5%), and a molecular class A carbapenem-hydrolyzing enzyme was detected at 1 site (4%). No class B or D carbapenem-hydrolyzing enzymes were detected. ESBLs and imported AmpC ß-lactamases were detected at a significant number of sites, indicating widespread penetration of these enzymes into U.S. medical institutions. Because these enzymes may significantly affect therapeutic outcomes, it is vital that clinical laboratories be aware of them and be able to detect their occurrence.

* Corresponding author. Mailing address: Center for Research in Antiinfectives and Biotechnology, Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, NE 68178. Phone: (402) 280-2921. Fax: (402) 280-1225. E-mail: kstaac{at}creighton.edu.

Antimicrobial Agents and Chemotherapy, December 2002, p. 3837-3842, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3837-3842.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jacoby, G. A. (2009). AmpC {beta}-Lactamases. Clin. Microbiol. Rev. 22: 161-182 [Abstract] [Full Text]  
  • Marschall, J., Tibbetts, R. J., Dunne, W. M. Jr., Frye, J. G., Fraser, V. J., Warren, D. K. (2009). Presence of the KPC Carbapenemase Gene in Enterobacteriaceae Causing Bacteremia and Its Correlation with In Vitro Carbapenem Susceptibility. J. Clin. Microbiol. 47: 239-241 [Abstract] [Full Text]  
  • Walther-Rasmussen, J., Hoiby, N. (2007). Class A carbapenemases. J Antimicrob Chemother 60: 470-482 [Abstract] [Full Text]  
  • Yang, K., Guglielmo, B J. (2007). Diagnosis and Treatment of Extended-Spectrum and AmpC {beta}-Lactamase-Producing Organisms. The Annals of Pharmacotherapy 41: 1427-1435 [Abstract] [Full Text]  
  • Moland, E. S., Hanson, N. D., Black, J. A., Hossain, A., Song, W., Thomson, K. S. (2006). Prevalence of Newer {beta}-Lactamases in Gram-Negative Clinical Isolates Collected in the United States from 2001 to 2002.. J. Clin. Microbiol. 44: 3318-3324 [Abstract] [Full Text]  
  • Verdet, C., Benzerara, Y., Gautier, V., Adam, O., Ould-Hocine, Z., Arlet, G. (2006). Emergence of DHA-1-Producing Klebsiella spp. in the Parisian Region: Genetic Organization of the ampC and ampR Genes Originating from Morganella morganii. Antimicrob. Agents Chemother. 50: 607-617 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Song, W., Moland, E. S., Hanson, N. D., Lewis, J. S., Jorgensen, J. H., Thomson, K. S. (2005). Failure of Cefepime Therapy in Treatment of Klebsiella pneumoniae Bacteremia. J. Clin. Microbiol. 43: 4891-4894 [Abstract] [Full Text]  
  • Black, J. A., Moland, E. S., Thomson, K. S. (2005). AmpC Disk Test for Detection of Plasmid-Mediated AmpC {beta}-Lactamases in Enterobacteriaceae Lacking Chromosomal AmpC {beta}-Lactamases. J. Clin. Microbiol. 43: 3110-3113 [Abstract] [Full Text]  
  • Bratu, S., Tolaney, P., Karumudi, U., Quale, J., Mooty, M., Nichani, S., Landman, D. (2005). Carbapenemase-producing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents. J Antimicrob Chemother 56: 128-132 [Abstract] [Full Text]  
  • Bratu, S., Landman, D., Haag, R., Recco, R., Eramo, A., Alam, M., Quale, J. (2005). Rapid Spread of Carbapenem-Resistant Klebsiella pneumoniae in New York City: A New Threat to Our Antibiotic Armamentarium. Arch Intern Med 165: 1430-1435 [Abstract] [Full Text]  
  • Woodford, N., Tierno, P. M. Jr., Young, K., Tysall, L., Palepou, M.-F. I., Ward, E., Painter, R. E., Suber, D. F., Shungu, D., Silver, L. L., Inglima, K., Kornblum, J., Livermore, D. M. (2004). Outbreak of Klebsiella pneumoniae Producing a New Carbapenem- Hydrolyzing Class A {beta}-Lactamase, KPC-3, in a New York Medical Center. Antimicrob. Agents Chemother. 48: 4793-4799 [Abstract] [Full Text]  
  • Literacka, E., Empel, J., Baraniak, A., Sadowy, E., Hryniewicz, W., Gniadkowski, M. (2004). Four Variants of the Citrobacter freundii AmpC-Type Cephalosporinases, Including Novel Enzymes CMY-14 and CMY-15, in a Proteus mirabilis Clone Widespread in Poland. Antimicrob. Agents Chemother. 48: 4136-4143 [Abstract] [Full Text]  
  • Liebana, E., Batchelor, M., Clifton-Hadley, F. A., Davies, R. H., Hopkins, K. L., Threlfall, E. J. (2004). First Report of Salmonella Isolates with the DHA-1 AmpC {beta}-Lactamase in the United Kingdom. Antimicrob. Agents Chemother. 48: 4492-4492 [Full Text]  
  • Poirel, L., Heritier, C., Spicq, C., Nordmann, P. (2004). In Vivo Acquisition of High-Level Resistance to Imipenem in Escherichia coli. J. Clin. Microbiol. 42: 3831-3833 [Abstract] [Full Text]  
  • Bonnefoy, A., Dupuis-Hamelin, C., Steier, V., Delachaume, C., Seys, C., Stachyra, T., Fairley, M., Guitton, M., Lampilas, M. (2004). In vitro activity of AVE1330A, an innovative broad-spectrum non-{beta}-lactam {beta}-lactamase inhibitor. J Antimicrob Chemother 54: 410-417 [Abstract] [Full Text]  
  • Alvarez, M., Tran, J. H., Chow, N., Jacoby, G. A. (2004). Epidemiology of Conjugative Plasmid-Mediated AmpC {beta}-Lactamases in the United States. Antimicrob. Agents Chemother. 48: 533-537 [Abstract] [Full Text]  
  • Paterson, D. L., Hujer, K. M., Hujer, A. M., Yeiser, B., Bonomo, M. D., Rice, L. B., Bonomo, R. A., the International Klebsiella Study Group,, (2003). Extended-Spectrum {beta}-Lactamases in Klebsiella pneumoniae Bloodstream Isolates from Seven Countries: Dominance and Widespread Prevalence of SHV- and CTX-M-Type {beta}-Lactamases. Antimicrob. Agents Chemother. 47: 3554-3560 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»