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Antimicrobial Agents and Chemotherapy, December 2002, p. 3981-3983, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3981-3983.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biochemical Characterization of TEM-92 Extended-Spectrum ß-Lactamase, a Protein Differing from TEM-52 in the Signal Peptide

Mariagrazia Perilli,1 Bernardetta Segatore,1 Maria Rosaria De Massis,1 Laura Pagani,2 Francesco Luzzaro,3 Gian Maria Rossolini,4 and Gianfranco Amicosante1*

Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, I-67100, L'Aquila,1 Dipartimento di Scienze Morfologiche, Eidologiche e Cliniche, Sezione di Microbiologia, Università di Pavia, I-27100, Pavia,2 Ospedale di Circolo di Varese, I-21100, Varese,3 Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, I-53100, Siena, Italy4

Received 15 May 2002/ Returned for modification 20 July 2002/ Accepted 27 August 2002

A blaTEM-92 gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.

* Corresponding author. Mailing address: Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Cattedra di Biochimica Clinica, Loc. Coppito, I-67100, L'Aquila, Italy. Phone: 39 0862 433455. Fax: 39 0862 433433. E-mail: amicosante{at}cc.univaq.it.

Antimicrobial Agents and Chemotherapy, December 2002, p. 3981-3983, Vol. 46, No. 12
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.12.3981-3983.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Endimiani, A., Luzzaro, F., Brigante, G., Perilli, M., Lombardi, G., Amicosante, G., Rossolini, G. M., Toniolo, A. (2005). Proteus mirabilis Bloodstream Infections: Risk Factors and Treatment Outcome Related to the Expression of Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 49: 2598-2605 [Abstract] [Full Text]  


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