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Antimicrobial Agents and Chemotherapy, February 2002, p. 438-442, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.438-442.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Mutations Affecting the Rossman Fold of Isoleucyl-tRNA Synthetase Are Correlated with Low-Level Mupirocin Resistance in Staphylococcus aureus

Martin Antonio,1 Neil McFerran,2 and Mark J. Pallen1*

Microbial Genomics and Pathogenesis Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, BT15 2TT,1 Department of Biochemistry, The Queen's University of Belfast, Belfast, BT12 6BN, United Kingdom2

Received 13 June 2001/ Returned for modification 15 August 2001/ Accepted 31 October 2001

The isoleucyl-tRNA synthetase (ileS) gene was sequenced in toto from 9 and in part from 31 Staphylococcus aureus strains with various degrees of susceptibility to mupirocin. All strains for which the mupirocin MIC was greater than 8 µg/ml contained point mutations affecting the Rossman fold via Val-to-Phe changes at either residue 588 (V588F) or residue 631 (V631F). The importance of the V588F mutation was confirmed by an allele-specific PCR survey of 32 additional strains. Additional mutations of uncertain significance were found in residues clustered on the surface of the IleS protein.

* Corresponding author. Mailing address: Microbial Genomics and Pathogenesis Unit, Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham BT15 2TT, United Kingdom. Phone: 44 (0)121 414 3344. Fax: 44 (0)121 414 3971. E-mail: m.j.pallen{at}bham.ac.uk.

Antimicrobial Agents and Chemotherapy, February 2002, p. 438-442, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.438-442.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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