This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beard, S. J.
Right arrow Articles by MacGowan, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beard, S. J.
Right arrow Articles by MacGowan, A. P.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, February 2002, p. 538-542, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.538-542.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Expression of lux Genes in a Clinical Isolate of Streptococcus pneumoniae: Using Bioluminescence To Monitor Gemifloxacin Activity

S. J. Beard,1 V. Salisbury,1*,{dagger} R. J. Lewis,1 J. A. Sharpe,2 and A. P. MacGowan3

Faculty of Applied Sciences, University of the West of England,1 Bristol Centre for Antimicrobial Research and Evaluation, Southmead Hospital, Bristol,3 School of Medicine, University of Leicester, Leicester, United Kingdom2

Received 7 May 2001/ Returned for modification 9 September 2001/ Accepted 1 November 2001

A clinical isolate of Streptococcus pneumoniae was transformed with a plasmid containing the lux operon of Photorhabdus luminescens that had been modified to function in gram-positive bacteria. Cells containing this plasmid produced light stably and constitutively, without compromising the growth rate. Light output was correlated with measurements of optical density and viable counts during exponential growth and provided a sensitive, real-time measure of the pharmacodynamics of the fluoroquinolone gemifloxacin.

* Corresponding author. Mailing address: Faculty of Applied Sciences, University of the West of England, Coldharbour La., Bristol BS16 1QY, United Kingdom. Phone: 44 117 3442492. Fax: 44 117 3442904. E-mail: vyv.salisbury{at}uwe.ac.uk.

{dagger} Present address: School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom.

Antimicrobial Agents and Chemotherapy, February 2002, p. 538-542, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.538-542.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mesak, L. R., Miao, V., Davies, J. (2008). Effects of Subinhibitory Concentrations of Antibiotics on SOS and DNA Repair Gene Expression in Staphylococcus aureus. Antimicrob. Agents Chemother. 52: 3394-3397 [Abstract] [Full Text]  
  • Biswas, I., Jha, J. K., Fromm, N. (2008). Shuttle expression plasmids for genetic studies in Streptococcus mutans. Microbiology 154: 2275-2282 [Abstract] [Full Text]  
  • Thorn, R. M. S., Nelson, S. M., Greenman, J. (2007). Use of a Bioluminescent Pseudomonas aeruginosa Strain within an In Vitro Microbiological System, as a Model of Wound Infection, To Assess the Antimicrobial Efficacy of Wound Dressings by Monitoring Light Production. Antimicrob. Agents Chemother. 51: 3217-3224 [Abstract] [Full Text]  
  • Vesterlund, S., Karp, M., Salminen, S., Ouwehand, A. C. (2006). Staphylococcus aureus adheres to human intestinal mucus but can be displaced by certain lactic acid bacteria. Microbiology 152: 1819-1826 [Abstract] [Full Text]  
  • Ibrahim, Y. M., Kerr, A. R., McCluskey, J., Mitchell, T. J. (2004). Control of Virulence by the Two-Component System CiaR/H Is Mediated via HtrA, a Major Virulence Factor of Streptococcus pneumoniae. J. Bacteriol. 186: 5258-5266 [Abstract] [Full Text]  
  • Ibrahim, Y. M., Kerr, A. R., McCluskey, J., Mitchell, T. J. (2004). Role of HtrA in the Virulence and Competence of Streptococcus pneumoniae. Infect. Immun. 72: 3584-3591 [Abstract] [Full Text]  
  • Vlasova, I. I., Asrieli, T. V., Gavrilova, E. M., Danilov, V. S. (2004). New Approach for Specific Determination of Antibiotics by Use of Luminescent Escherichia coli and Immune Serum. Appl. Environ. Microbiol. 70: 1245-1248 [Abstract] [Full Text]  
  • Alloush, H. M., Salisbury, V., Lewis, R. J., MacGowan, A. P. (2003). Pharmacodynamics of linezolid in a clinical isolate of Streptococcus pneumoniae genetically modified to express lux genes. J Antimicrob Chemother 52: 511-513 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»