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Antimicrobial Agents and Chemotherapy, February 2002, p. 566-569, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.566-569.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Integron-Located oxa-32 Gene Cassette Encoding an Extended-Spectrum Variant of OXA-2 ß-Lactamase from Pseudomonas aeruginosa

Laurent Poirel,1 Patrick Gerome,2 Christophe De Champs,1,3 Jean Stephanazzi,4 Thierry Naas,1 and Patrice Nordmann1*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre,1 Service de Biologie,2 Centre de Traitement des Brûlés, Hôpital d’Instruction des Armées Percy, 92140 Clamart,4 Laboratoire de Bactériologie, Faculté de Médecine, Université d’Auvergne 63001 Clermont-Ferrand, France3

Received 8 November 2000/ Returned for modification 19 March 2001/ Accepted 26 October 2001

Pseudomonas aeruginosa clinical isolate CY-1, which was resistant to ceftazidime, harbored a conjugative ca. 250-kb plasmid that contained a class 1 integron with two gene cassettes encoding OXA-32, an OXA-2- type ß-lactamase, and the aminoglycoside acetyltransferase AAC(6')Ib9. OXA-32 differed from OXA-2 by an Leu169Ile amino acid substitution (class D numbering). Site-directed mutagenesis established that Ile169 is responsible for resistance to ceftazidime but not to cefotaxime.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. Email: nordmann.patrice{at}bct.ap-hop-paris.fr.

Antimicrobial Agents and Chemotherapy, February 2002, p. 566-569, Vol. 46, No. 2
0066-4804/01/$04.00+0     DOI: 10.1128/AAC.46.2.566-569.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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