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Antimicrobial Agents and Chemotherapy, May 2002, p. 1546-1549, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1546-1549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

30S Ribosomal Subunit Assembly Is a Target for Inhibition by Aminoglycosides in Escherichia coli

Roopal Mehta and W. Scott Champney*

Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee 37614

Received 13 September 2001/ Returned for modification 16 November 2001/ Accepted 24 January 2002

The aminoglycosides paromomycin and neomycin were examined in Escherichia coli cells for an inhibitory effect on 30S ribosomal subunit assembly. Both compounds inhibited the growth rate, viable cell number, and protein synthesis rate with similar 50% inhibitory concentrations. Each drug also showed a concentration-dependent inhibition of 30S subunit formation. The inhibitory effect on 30S particle formation was approximately equivalent to the inhibitory effect on translation for these antibiotics.

* Department of Biochemistry and Molecular Biology, J. H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614. Phone: (423) 439-4651. Fax: (423) 439-8235. E-mail: champney{at}etsu.edu.

Antimicrobial Agents and Chemotherapy, May 2002, p. 1546-1549, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1546-1549.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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