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Antimicrobial Agents and Chemotherapy, May 2002, p. 1568-1570, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1568-1570.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Lack of Interaction of Fluoroquinolones with Lipopolysaccharides

B. Lindner,1 A. Wiese,1 K. Brandenburg,1 U. Seydel,1 and A. Dalhoff2*

Division of Biophysics, Research Center Borstel, Borstel,1 Bayer AG, Wuppertal, Germany2

Received 5 April 2001/ Returned for modification 19 August 2001/ Accepted 25 January 2002

Fluoroquinolones are known to chelate with di- and trivalent cations, and it has accordingly been claimed that they perturb the integrity of the outer membrane (OM) of gram-negative bacteria. So far, chelation has not been assessed in biologically relevant test systems. Therefore, we investigated the interaction of ciprofloxacin and moxifloxacin in the absence and presence of Mg2+ with whole bacteria and isolated lipopolysaccharide (LPS) from various rough mutant strains of Salmonella enterica chemotypes by applying different biophysical techniques. We found that the fluoroquinolones did not disturb the integrity of the OM and neither were incorporated into LPS monolayers nor displaced Ca2+ from LPS monolayers, suggesting that chelation of fluoroquinolones with divalent cations does not contribute to the antibacterial effect of fluoroquinolones.

* Corresponding author. Mailing address: Bayer AG, Pharma Research Center, Aprather Weg, D-42113 Wuppertal, Germany. Phone: 49 (0) 202-368239. Fax: 49 (0) 202-364144/45. E-mail: axel.dalhoff.ad{at}bayer-ag.de.

Antimicrobial Agents and Chemotherapy, May 2002, p. 1568-1570, Vol. 46, No. 5
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.5.1568-1570.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Campos, M. A., Morey, P., Bengoechea, J. A. (2006). Quinolones sensitize gram-negative bacteria to antimicrobial peptides.. Antimicrob. Agents Chemother. 50: 2361-2367 [Abstract] [Full Text]  


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