This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Steinstraesser, L. Articles by Wang, S. C. Search for Related Content PubMed PubMed Citation Articles by Steinstraesser, L. Articles by Wang, S. C.

 Previous Article  |  Next Article 

Antimicrobial Agents and Chemotherapy, June 2002, p. 1837-1844, Vol. 46, No. 6
0066-4804/02/$04.00+0     DOI: 10.1128/AAC.46.6.1837-1844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Activity of Novispirin G10 against Pseudomonas aeruginosa In Vitro and in Infected Burns

Departments of Surgery,¹ Medicine,⁶ Pathology, University of Michigan, Ann Arbor, Michigan,⁵ Department of Microbiology, University of Iowa, Ames, Iowa,³ Department of Medicine, UCLA School of Medicine, Los Angeles, California,⁴ Department of Plastic and Reconstructive Surgery, Ruhr University Bergmannsheil, Bochum, Germany²

Received 13 April 2001/ Returned for modification 11 June 2001/ Accepted 7 March 2002

The emergence of multidrug-resistant microbes has serious implications for managing infection and sepsis and has stimulated efforts to develop alternative treatments, such as antimicrobial peptides. The objective of this study was to test a designer peptide, novispirin G10, against multidrug-resistant microorganisms. By two-stage radial diffusion assays, its activity against such organisms compared favorably with that of standard antibiotics and other antimicrobial peptides. It killed bacteria very rapidly, was nonhemolytic, and was relatively noncytotoxic. The peptide induced an immediate, massive efflux of potassium from Pseudomonas aeruginosa, suggesting that it altered the permeability of its inner membrane. The presence of human serum reduced but did not eliminate its activity. We tested the in vivo activity of novispirin G10 in rats with an infected, partial-thickness burn that covered 20% of their total body surface area. The burned area was seeded with 10⁶ CFU of a Silvadene-resistant P. aeruginosa strain, and 24 h later a single treatment with 0, 1, 3, or 6 mg of synthetic novispirin G10 (n = 16 at each concentration) per kg was given intradermally. Significant bacterial killing (P < 0.0001) was evident within 4 h in each peptide group compared to controls receiving vehicle. Antimicrobial peptides such as novispirin G10 may provide a useful alternative or adjunct to standard antibiotic agents in treating burns or other wound infections.

This article has been cited by other articles:

• Kobayashi, M., Yoshida, T., Takeuchi, D., Jones, V. C., Shigematsu, K., Herndon, D. N., Suzuki, F. (2008). Gr-1+CD11b+ cells as an accelerator of sepsis stemming from Pseudomonas aeruginosa wound infection in thermally injured mice. J. Leukoc. Biol. 83: 1354-1362 [Abstract] [Full Text]  
• Jacobsen, F., Mohammadi-Tabrisi, A., Hirsch, T., Mittler, D., Mygind, P. H., Sonksen, C. P., Raventos, D., Kristensen, H. H., Gatermann, S., Lehnhardt, M., Daigeler, A., Steinau, H. U., Steinstraesser, L. (2007). Antimicrobial activity of the recombinant designer host defence peptide P-novispirin G10 in infected full-thickness wounds of porcine skin. J Antimicrob Chemother 59: 493-498 [Abstract] [Full Text]  
• Benincasa, M., Scocchi, M., Pacor, S., Tossi, A., Nobili, D., Basaglia, G., Busetti, M., Gennaro, R. (2006). Fungicidal activity of five cathelicidin peptides against clinically isolated yeasts. J Antimicrob Chemother 58: 950-959 [Abstract] [Full Text]  
• Eckert, R., Brady, K. M., Greenberg, E. P., Qi, F., Yarbrough, D. K., He, J., McHardy, I., Anderson, M. H., Shi, W. (2006). Enhancement of Antimicrobial Activity against Pseudomonas aeruginosa by Coadministration of G10KHc and Tobramycin. Antimicrob. Agents Chemother. 50: 3833-3838 [Abstract] [Full Text]  
• Eckert, R., Qi, F., Yarbrough, D. K., He, J., Anderson, M. H., Shi, W. (2006). Adding Selectivity to Antimicrobial Peptides: Rational Design of a Multidomain Peptide against Pseudomonas spp.. Antimicrob. Agents Chemother. 50: 1480-1488 [Abstract] [Full Text]  
• Song, Z., Wu, H., Mygind, P., Raventos, D., Sonksen, C., Kristensen, H.-H., Hoiby, N. (2005). Effects of Intratracheal Administration of Novispirin G10 on a Rat Model of Mucoid Pseudomonas aeruginosa Lung Infection. Antimicrob. Agents Chemother. 49: 3868-3874 [Abstract] [Full Text]  
• Viejo-Diaz, M., Andres, M. T., Fierro, J. F. (2005). Different Anti-Candida Activities of Two Human Lactoferrin-Derived Peptides, Lfpep and Kaliocin-1. Antimicrob. Agents Chemother. 49: 2583-2588 [Abstract] [Full Text]  
• Jacobsen, F., Baraniskin, A., Mertens, J., Mittler, D., Mohammadi-Tabrisi, A., Schubert, S., Soltau, M., Lehnhardt, M., Behnke, B., Gatermann, S., Steinau, H. U., Steinstraesser, L. (2005). Activity of histone H1.2 in infected burn wounds. J Antimicrob Chemother 55: 735-741 [Abstract] [Full Text]  
• Faber, C., Hoogendoorn, R. J. W., Stallmann, H. P., Lyaruu, D. M., van Nieuw Amerongen, A., Wuisman, P. I. J. M., on behalf of STEGA (Skeletal Tissue Engineering Gr, (2004). In vivo comparison of Dhvar-5 and gentamicin in an MRSA osteomyelitis prevention model. J Antimicrob Chemother 54: 1078-1084 [Abstract] [Full Text]  
• Stallmann, H. P., Faber, C., Bronckers, A. L. J. J., Nieuw Amerongen, A. V., Wuisman, P. I. J. M. (2004). Osteomyelitis prevention in rabbits using antimicrobial peptide hLF1-11- or gentamicin-containing calcium phosphate cement. J Antimicrob Chemother 54: 472-476 [Abstract] [Full Text]  
• Bartlett, K. H., McCray, P. B. Jr., Thorne, P. S. (2003). Novispirin G10-Induced Lung Toxicity in a Klebsiella pneumoniae Infection Model. Antimicrob. Agents Chemother. 47: 3901-3906 [Abstract] [Full Text]